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Mouse Anti-HEPH Recombinant Antibody (CBFYH-0931) (CBMAB-H1864-FY)


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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
CBFYH-0931
Antibody Isotype
IgG2a
Application
WB, IHC-Fr, IF

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
hephaestin
Introduction
This gene encodes a member of the multicopper oxidase protein family. The encoded protein is involved in the transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system, and may be involved in copper transport and homeostasis. In mouse, defects in this gene can lead to severe microcytic anemia. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human9843
Mouse15203
Rat117240
UniProt ID
HumanQ9BQS7
MouseQ9Z0Z4
RatQ920H8
Alternative Names
Hephaestin; EC 1.16.3.1; EC 1.-.-.-; KIAA0698; CPL
Function
May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.
Biological Process
Cellular iron ion homeostasis Source: InterPro
Copper ion transport Source: UniProtKB-KW
Iron ion homeostasis Source: GO_Central
Iron ion transport Source: UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 24-1110
Helical: 1111-1131
Cytoplasmic: 1132-1158
More Infomation

Ramavath, H. N., Konda, V., & Pullakhandam, R. (2023). Quercetin Inhibits Hephaestin Expression and Iron Transport in Intestinal Cells: Possible Role of PI3K Pathway. Nutrients, 15(5), 1205.

Ramavath, H. N., Mashurabad, P. C., Yaduvanshi, P. S., Veleri, S., Sharp, P. A., & Pullakhandam, R. (2022). Zinc induces hephaestin expression via a PI3K-CDX2 dependent mechanism to regulate iron transport in intestinal Caco-2 cells. Biochemical and Biophysical Research Communications, 626, 1-7.

Zacchi, P., Belmonte, B., Mangogna, A., Morello, G., Scola, L., Martorana, A., & Borelli, V. (2021). The ferroxidase hephaestin in lung cancer: pathological significance and prognostic value. Frontiers in oncology, 11, 638856.

Dlouhy, A. C., Bailey, D. K., Steimle, B. L., Parker, H. V., & Kosman, D. J. (2019). Fluorescence resonance energy transfer links membrane ferroportin, hephaestin but not ferroportin, amyloid precursor protein complex with iron efflux. Journal of Biological Chemistry, 294(11), 4202-4214.

Fuqua, B. K., Lu, Y., Frazer, D. M., Darshan, D., Wilkins, S. J., Dunn, L., ... & Anderson, G. J. (2018). Severe iron metabolism defects in mice with double knockout of the multicopper ferroxidases hephaestin and ceruloplasmin. Cellular and molecular gastroenterology and hepatology, 6(4), 405-427.

Zheng, J., Chen, M., Liu, G., Xu, E., & Chen, H. (2018). Ablation of hephaestin and ceruloplasmin results in iron accumulation in adipocytes and type 2 diabetes. FEBS letters, 592(3), 394-401.

Chen, M., Zheng, J., Liu, G., Xu, E., Wang, J., Fuqua, B. K., ... & Chen, H. (2018). Ceruloplasmin and hephaestin jointly protect the exocrine pancreas against oxidative damage by facilitating iron efflux. Redox Biology, 17, 432-439.

Xu, E., Chen, M., Zheng, J., Maimaitiming, Z., Zhong, T., & Chen, H. (2018). Deletion of hephaestin and ceruloplasmin induces a serious systemic iron deficiency and disrupts iron homeostasis. Biochemical and biophysical research communications, 503(3), 1905-1910.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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