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Mouse Anti-HHIP Recombinant Antibody (5D11) (CBMAB-A3889-LY)

The product is antibody recognizes HHIP. The antibody 5D11 immunoassay techniques such as: WB, ELISA.
See all HHIP antibodies
Published Data
Fig.1 Genes associated with cell growth that demonstrated significant differential expression (p b 0.05) after HHIP-knock-down in Beas-2B (A) or normal human bronchial epithelial cells (NHBE) (B).
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Fig.2 The Hedgehog-binding domain is required for the inhibition of the Shh response by Hhip.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
5D11
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
HHIP (NP_071920, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
hedgehog interacting protein
Introduction
This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry. [provided by RefSeq]
Entrez Gene ID
64399
UniProt ID
Q96QV1
Alternative Names
FLJ20992; FLJ90230; HIP; STQTL12
Function
Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.
Biological Process
Dorsal/ventral pattern formation Source: Ensembl
Epithelial tube branching involved in lung morphogenesis Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of signal transduction Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: BHF-UCL
Neuroblast proliferation Source: Ensembl
Regulation of fibroblast growth factor receptor signaling pathway Source: Ensembl
Signal transduction Source: Ensembl
Skeletal system morphogenesis Source: BHF-UCL
Cellular Location
Cell membrane; Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment.
Isoform 2: Cytoplasm

Caligiuri, S. P., Howe, W. M., Wills, L., Smith, A. C., Lei, Y., Bali, P., ... & Kenny, P. J. (2022). Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences, 119(46), e2209870119.

Griffiths, S. C., Schwab, R. A., El Omari, K., Bishop, B., Iverson, E. J., Malinauskas, T., ... & Siebold, C. (2021). Hedgehog-Interacting Protein is a multimodal antagonist of Hedgehog signalling. Nature communications, 12(1), 7171.

Li, P., Zhang, X., Murphy, A. J., Costa, M., Zhao, X., & Sun, H. (2021). Downregulation of hedgehog-interacting protein (HHIP) contributes to hexavalent chromium-induced malignant transformation of human bronchial epithelial cells. Carcinogenesis, 42(1), 136-147.

Li, Y., Zhang, L., Polverino, F., Guo, F., Hao, Y., Lao, T., ... & Zhou, X. (2021). Hedgehog interacting protein (HHIP) represses airway remodeling and metabolic reprogramming in COPD-derived airway smooth muscle cells. Scientific reports, 11(1), 9074.

Chou, H. W., Hung, H. C., Lin, C. H., Lin, A. C., Du, Y. F., Cheng, K. P., ... & Ou, H. Y. (2021). The serum concentrations of hedgehog-interacting protein, a novel biomarker, were decreased in overweight or obese subjects. Journal of Clinical Medicine, 10(4), 742.

Miyata, K. N., Zhao, X. P., Chang, S. Y., Liao, M. C., Lo, C. S., Chenier, I., ... & Zhang, S. L. (2020). Increased urinary excretion of hedgehog interacting protein (uHhip) in early diabetic kidney disease. Translational Research, 217, 1-10.

Lin, A. C., Hung, H. C., Chen, Y. W., Cheng, K. P., Li, C. H., Lin, C. H., ... & Ou, H. Y. (2019). Elevated Hedgehog-interacting protein levels in subjects with prediabetes and type 2 diabetes. Journal of Clinical Medicine, 8(10), 1635.

Nchienzia, H., Liao, M. C., Zhao, X. P., Chang, S. Y., Lo, C. S., Chenier, I., ... & Zhang, S. L. (2019). Hedgehog interacting protein (Hhip) regulates insulin secretion in mice fed high fat diets. Scientific Reports, 9(1), 11183.

Zhao, X. P., Chang, S. Y., Liao, M. C., Lo, C. S., Chenier, I., Luo, H., ... & Zhang, S. L. (2018). Hedgehog interacting protein promotes fibrosis and apoptosis in glomerular endothelial cells in murine diabetes. Scientific Reports, 8(1), 5958.

Sun, H., juan Ni, S., Ye, M., Weng, W., Zhang, Q., Zhang, M., ... & Sheng, W. (2018). Hedgehog interacting protein 1 is a prognostic marker and suppresses cell metastasis in gastric cancer. Journal of Cancer, 9(24), 4642.

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For research use only. Not intended for any clinical use.

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