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Mouse Anti-HIP1 Recombinant Antibody (1F12) (CBMAB-A3904-LY)

The product is antibody recognizes HIP1. The antibody 1F12 immunoassay techniques such as: WB, ELISA.
See all HIP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1F12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
HIP1 (NP_005329, 928 a.a. ~ 1037 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Huntingtin Interacting Protein 1
Introduction
The product of this gene is a membrane-associated protein that colocalizes with huntingtin. This protein has similarities to cytoskeleton proteins and its interaction with huntingtin is thought to play a functional role in the cell filament network. Loss of normal huntingtin-HIP1 interaction in Huntington disease may contribute to a defect in membrane-cytoskeletal integrity in the brain. This gene could help in the understanding of the normal function of huntingtin and also the pathogenesis of Huntington disease. It also has been implicated in the pathogenesis of hematopoietic malignancies. An alternative splice variant of this gene has been described but its full length sequence has not been determined. [provided by RefSeq]
Entrez Gene ID
3092
UniProt ID
O00291
Alternative Names
ILWEQ; MGC126506
Function
Plays a role in clathrin-mediated endocytosis and trafficking (PubMed:11532990, PubMed:11577110, PubMed:11889126).

Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (By similarity).

Regulates presynaptic nerve terminal activity (By similarity).

Enhances androgen receptor (AR)-mediated transcription (PubMed:16027218).

May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (PubMed:11007801).

Binds 3-phosphoinositides (via ENTH domain) (PubMed:14732715).

May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (PubMed:14732715).

May play a functional role in the cell filament networks (PubMed:18790740).

May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed:11007801, PubMed:12163454).
Biological Process
Actin filament organization Source: GO_Central
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
Apoptotic process Source: MGI
Apoptotic signaling pathway Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Clathrin coat assembly Source: UniProtKB
Clathrin-dependent endocytosis Source: InterPro
Endocytosis Source: GO_Central
Neurotransmitter receptor transport Source: ParkinsonsUK-UCL
Positive regulation of epidermal growth factor receptor signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of platelet-derived growth factor receptor-beta signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of protein kinase B signaling Source: ParkinsonsUK-UCL
Positive regulation of receptor-mediated endocytosis Source: UniProtKB
Protein stabilization Source: ParkinsonsUK-UCL
Regulation of apoptotic process Source: MGI
Regulation of endocytosis Source: ParkinsonsUK-UCL
Cellular Location
Nucleus; Cytoplasm; Endomembrane system; Clathrin-coated vesicle membrane. Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR.
Involvement in disease
A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB (PubMed:9616134). The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes (PubMed:9616134). The reciprocal PDGFRB-HIP1 transcript is not expressed (PubMed:9616134).

Prasad, P., Chongtham, J., Tripathi, S. C., Ganguly, N. K., Mittal, S. A., & Srivastava, T. (2023). Huntingtin Interacting Protein-1 expression is regulated via HIF2 axis in Lung Adenocarcinoma. bioRxiv, 2023-03.

Lacombe, L., Hovington, H., Brisson, H., Mehdi, S., Beillevaire, D., Émond, J. P., ... & Lévesque, E. (2023). UGT2B28 accelerates prostate cancer progression through stabilization of the endocytic adaptor protein HIP1 regulating AR and EGFR pathways. Cancer Letters, 553, 215994.

Surbhi, Goel, A., Chaturvedi, V., Verma, S., Rawat, S., Ganguly, N. K., & Mittal, S. A. (2022). Huntingtin Interacting Protein 1 (HIP1) autoantibodies as a novel potential surrogate marker for Rheumatoid Arthritis: Pilot Study. medRxiv, 2022-09.

Tamta, S., Goel, A., Chaturvedi, V., Verma, S., Rawat, S., Ganguly, N. K., & Mittal, S. A. (2022). Huntingtin Interacting Protein 1 (HIP1) autoantibodies as a novel potential surrogate marker for Rheumatoid Arthritis: Pilot Study.

Kang, J., Deng, Q. M., Peng, K. C., Li, P., Zhu, B. T., Wang, P., ... & Yang, J. J. (2022). Clinicopathological features and resistance mechanisms in HIP1‐ALK‐rearranged lung cancer: A multicenter study. Genes, Chromosomes and Cancer, 61(4), 177-186.

Murakami, T., Matsuura, R., Chutiwitoonchai, N., Takei, M., & Aida, Y. (2021). Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages. Viruses, 13(11), 2308.

Sun, Y., Zhou, Y., Xia, J., Wen, M., Wang, X., Zhang, J., ... & Jiang, T. (2021). Abnormally high HIP1 expression is associated with metastatic behaviors and poor prognosis in ESCC. Oncology Letters, 21(2), 1-1.

Laragione, T., Brenner, M., Lahiri, A., Gao, E., Harris, C., & Gulko, P. S. (2018). Huntingtin-interacting protein 1 (HIP1) regulates arthritis severity and synovial fibroblast invasiveness by altering PDGFR and Rac1 signalling. Annals of the rheumatic diseases, 77(11), 1627-1635.

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For research use only. Not intended for any clinical use.

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