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Mouse Anti-HMG20B Recombinant Antibody (1F6) (CBMAB-A3955-LY)

The product is antibody recognizes HMG20B. The antibody 1F6 immunoassay techniques such as: WB, ELISA.
See all HMG20B antibodies
Published Data
Fig. 1 Hmg20b knockdown in I/11 cells was mediated with two different shRNA constructs. Whole cell lysates were prepared and used for Western blot analysis of Hmg20b protein. Cyclin D3 served as a loading control.
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Fig. 2 Confocal images of control HeLa cells stained with anti-HMG20b monoclonal antibody 1F6 (green). Scale bar is 5 μm.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1F6
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
HMG20B (NP_006330, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
High Mobility Group 20B
Entrez Gene ID
10362
UniProt ID
Q9P0W2
Alternative Names
BRAF25; BRAF35; FLJ26127; HMGX2; HMGXB2; PP7706; SMARCE1r; SOXL; pp8857
Function
Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
Biological Process
Cell cycle Source: UniProtKB-KW
Chromatin organization Source: UniProtKB-KW
Negative regulation of protein sumoylation Source: Ensembl
Positive regulation of neuron differentiation Source: Ensembl
Regulation of gene expression Source: GO_Central
Skeletal muscle cell differentiation Source: Ensembl
Cellular Location
Nucleus; Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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