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Rabbit Anti-HMGN1 Recombinant Antibody (D1I5O) (CBMAB-H2527-FY)

This product is rabbit antibody that recognizes HMGN1. The antibody D1I5O can be used for immunoassay techniques such as: WB, IP, IF.
See all HMGN1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
D1I5O
Application
WB, IP, IF

Basic Information

Specificity
Human, Monkey
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
high mobility group nucleosome binding domain 1
Introduction
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes.
Entrez Gene ID
Human3150
Monkey722589
UniProt ID
HumanP05114
MonkeyA0A0D9R7M9
Alternative Names
High Mobility Group Nucleosome Binding Domain 1; High Mobility Group Nucleosome-Binding Domain-Containing Protein 1; High-Mobility Group (Nonhistone Chromosomal) Protein 14; High-Mobility Group Nucleosome Binding 1; Nonhistone Chromosomal Protein HMG-14; HMG14; High-Mobility Group Nucleosome Binding Domain 1; Non-Histone Chromosomal Protein HMG-14
Function
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
Biological Process
Chromatin organization Source: GO_Central
Positive regulation of DNA-templated transcription, elongation Source: ProtInc
Cellular Location
Nucleus; Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
PTM
Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.

Yang, C., Ma, Z., Wang, K., Dong, X., Huang, M., Li, Y., ... & Zhang, X. (2023). HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing. Nature Communications, 14(1), 2430.

Mao, Y., Yu, J., Da, J., Yu, F., & Zha, Y. (2023). Acteoside alleviates UUO-induced inflammation and fibrosis by regulating the HMGN1/TLR4/TREM1 signaling pathway. PeerJ, 11, e14765.

Page, E. C., Heatley, S. L., Eadie, L. N., McClure, B. J., de Bock, C. E., Omari, S., ... & White, D. L. (2022). HMGN1 plays a significant role in CRLF2 driven Down Syndrome leukemia and provides a potential therapeutic target in this high-risk cohort. Oncogene, 41(6), 797-808.

Farley, S. J., Grishok, A., & Zeldich, E. (2022). Shaking up the silence: consequences of HMGN1 antagonizing PRC2 in the Down syndrome brain. Epigenetics & Chromatin, 15(1), 1-27.

Jiang, M., Liu, J., Yang, D., Tross, D., Li, P., Chen, F., ... & Chen, X. (2021). A TNFR2 antibody by countering immunosuppression cooperates with HMGN1 and R848 immune stimulants to inhibit murine colon cancer. International Immunopharmacology, 101, 108345.

Chen, C. Y., Ueha, S., Ishiwata, Y., Shichino, S., Yokochi, S., Yang, D., ... & Matsushima, K. (2021). Combining an alarmin HMGN1 peptide with PD-L1 blockade results in robust antitumor effects with a concomitant increase of stem-like/progenitor exhausted CD8+ T cells. Cancer immunology research, 9(10), 1214-1228.

Mehnert, M., Li, W., Wu, C., Salovska, B., & Liu, Y. (2019). Combining rapid data independent acquisition and CRISPR gene deletion for studying potential protein functions: a case of HMGN1. Proteomics, 19(13), 1800438.

Mowery, C. T., Reyes, J. M., Cabal-Hierro, L., Higby, K. J., Karlin, K. L., Wang, J. H., ... & Lane, A. A. (2018). Trisomy of a Down syndrome critical region globally amplifies transcription via HMGN1 overexpression. Cell reports, 25(7), 1898-1911.

Alam, M. M., Yang, D., Trivett, A., Meyer, T. J., & Oppenheim, J. J. (2018). HMGN1 and R848 synergistically activate dendritic cells using multiple signaling pathways. Frontiers in immunology, 9, 2982.

Yang, D., Han, Z., Alam, M. M., & Oppenheim, J. J. (2018, August). High-mobility group nucleosome binding domain 1 (HMGN1) functions as a Th1-polarizing alarmin. In Seminars in immunology (Vol. 38, pp. 49-53). Academic Press.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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