Human HMGN1 ELISA Kit (V2LY-0626-LY3671)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0051 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
10.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
high mobility group nucleosome binding domain 1
Function
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity).
Biological Process
Chromatin organization Source: GO_Central
Positive regulation of DNA-templated transcription, elongation Source: ProtInc
Cellular Location
Nucleus; Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
PTM
Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.

Yang, C., Ma, Z., Wang, K., Dong, X., Huang, M., Li, Y., ... & Zhang, X. (2023). HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing. Nature Communications, 14(1), 2430.

Mao, Y., Yu, J., Da, J., Yu, F., & Zha, Y. (2023). Acteoside alleviates UUO-induced inflammation and fibrosis by regulating the HMGN1/TLR4/TREM1 signaling pathway. PeerJ, 11, e14765.

Page, E. C., Heatley, S. L., Eadie, L. N., McClure, B. J., de Bock, C. E., Omari, S., ... & White, D. L. (2022). HMGN1 plays a significant role in CRLF2 driven Down Syndrome leukemia and provides a potential therapeutic target in this high-risk cohort. Oncogene, 41(6), 797-808.

Farley, S. J., Grishok, A., & Zeldich, E. (2022). Shaking up the silence: consequences of HMGN1 antagonizing PRC2 in the Down syndrome brain. Epigenetics & Chromatin, 15(1), 1-27.

Jiang, M., Liu, J., Yang, D., Tross, D., Li, P., Chen, F., ... & Chen, X. (2021). A TNFR2 antibody by countering immunosuppression cooperates with HMGN1 and R848 immune stimulants to inhibit murine colon cancer. International Immunopharmacology, 101, 108345.

Chen, C. Y., Ueha, S., Ishiwata, Y., Shichino, S., Yokochi, S., Yang, D., ... & Matsushima, K. (2021). Combining an alarmin HMGN1 peptide with PD-L1 blockade results in robust antitumor effects with a concomitant increase of stem-like/progenitor exhausted CD8+ T cells. Cancer immunology research, 9(10), 1214-1228.

Mehnert, M., Li, W., Wu, C., Salovska, B., & Liu, Y. (2019). Combining rapid data independent acquisition and CRISPR gene deletion for studying potential protein functions: a case of HMGN1. Proteomics, 19(13), 1800438.

Mowery, C. T., Reyes, J. M., Cabal-Hierro, L., Higby, K. J., Karlin, K. L., Wang, J. H., ... & Lane, A. A. (2018). Trisomy of a Down syndrome critical region globally amplifies transcription via HMGN1 overexpression. Cell reports, 25(7), 1898-1911.

Alam, M. M., Yang, D., Trivett, A., Meyer, T. J., & Oppenheim, J. J. (2018). HMGN1 and R848 synergistically activate dendritic cells using multiple signaling pathways. Frontiers in immunology, 9, 2982.

Yang, D., Han, Z., Alam, M. M., & Oppenheim, J. J. (2018, August). High-mobility group nucleosome binding domain 1 (HMGN1) functions as a Th1-polarizing alarmin. In Seminars in immunology (Vol. 38, pp. 49-53). Academic Press.

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For research use only. Not intended for any clinical use.

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