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Mouse Anti-HPRT1 (AA 1-218) Recombinant Antibody (5F11A7) (CBMAB-H2914-FY)

This product is mouse antibody that recognizes HPRT1. The antibody 5F11A7 can be used for immunoassay techniques such as: ELISA, FC, IHC, WB.
See all HPRT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5F11A7
Antibody Isotype
IgG1
Application
ELISA, FC, IHC, WB

Basic Information

Immunogen
Purified recombinant fragment of human HPRT1 expressed in E. Coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-218

Target

Full Name
hypoxanthine phosphoribosyltransferase 1
Introduction
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Entrez Gene ID
3251
UniProt ID
P00492
Alternative Names
Hypoxanthine Phosphoribosyltransferase 1; EC 2.4.2.8; HGPRTase; HGPRT; HPRT; Hypoxanthine-Guanine Phosphoribosyltransferase 1; Hypoxanthine-Guanine Phosphoribosyltransferase; Testicular Tissue Protein Li 89; Lesch-Nyhan Syndrome
Function
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Biological Process
Adenine metabolic process Source: Ensembl
AMP salvage Source: Ensembl
Central nervous system neuron development Source: Ensembl
Cerebral cortex neuron differentiation Source: Ensembl
Dendrite morphogenesis Source: Ensembl
Dopamine metabolic process Source: Ensembl
GMP catabolic process Source: UniProtKB
GMP salvage Source: MGI
Grooming behavior Source: Ensembl
Guanine salvage Source: UniProtKB
Hypoxanthine metabolic process Source: UniProtKB
Hypoxanthine salvage Source: UniProtKB
IMP metabolic process Source: UniProtKB
IMP salvage Source: GO_Central
Locomotory behavior Source: Ensembl
Lymphocyte proliferation Source: Ensembl
Positive regulation of dopamine metabolic process Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Purine nucleotide biosynthetic process Source: UniProtKB
Purine ribonucleoside salvage Source: UniProtKB
Response to amphetamine Source: Ensembl
Striatum development Source: Ensembl
T cell mediated cytotoxicity Source: Ensembl
Cellular Location
Cytoplasm
Involvement in disease
Lesch-Nyhan syndrome (LNS):
Characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.
Hyperuricemia, HPRT-related (HRH):
An X-linked metabolic disorder characterized by uric acid excess in the blood, renal stones, uric acid nephropathy, and renal obstruction. After puberty, the hyperuricemia may cause gout.

Wu, T., Jiao, Z., Li, Y., Su, X., Yao, F., Peng, J., ... & Yang, A. (2022). HPRT1 promotes chemoresistance in oral squamous cell carcinoma via activating MMP1/PI3K/Akt signaling pathway. Cancers, 14(4), 855.

Li, C., Chen, S., Jia, W., Li, W., Wei, D., Cao, S., ... & Lei, D. (2022). Identify metabolism-related genes IDO1, ALDH2, NCOA2, SLC7A5, SLC3A2, LDHB, and HPRT1 as potential prognostic markers and correlate with immune infiltrates in head and neck squamous cell carcinoma. Frontiers in immunology, 13, 955614.

Lee, S. Y., Choe, Y. H., Han, J. H., Hwang, G., Choi, M. Y., Thakur, G., ... & Hwang, T. S. (2022). HPRT1 Most Suitable Reference Gene for Accurate Normalization of mRNA Expression in Canine Dermal Tissues with Radiation Therapy. Genes, 13(11), 1928.

Ahmadi, M., Eftekhari Kenzerki, M., Akrami, S. M., Pashangzadeh, S., Hajiesmaeili, F., Rahnavard, S., ... & Mousavi, P. (2021). Overexpression of HPRT1 is associated with poor prognosis in head and neck squamous cell carcinoma. FEBS Open Bio, 11(9), 2525-2540.

Yang, Q., Humphreys, S. C., Lade, J. M., & Li, A. P. (2021). Prolonged cultured human hepatocytes as an in vitro experimental system for the evaluation of potency and duration of activity of RNA therapeutics: Demonstration of prolonged duration of gene silencing effects of a GalNAc-conjugated human hypoxanthine phosphoribosyl transferase (HPRT1) siRNA. Biochemical Pharmacology, 189, 114374.

Jiang, J., Piao, X., Hu, S., Gao, J., & Bao, M. (2020). LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/β-catenin signaling pathway. Aging (Albany NY), 12(10), 8820.

Jinnah, H. A. (2020). HPRT1 disorders.

De Mandal, S., Mazumder, T. H., Panda, A. K., Kumar, N. S., & Jin, F. (2020). Analysis of synonymous codon usage patterns of HPRT1 gene across twelve mammalian species. Genomics, 112(1), 304-311.

Agrahari, A. K., Priya, M. K., Kumar, M. P., Tayubi, I. A., Siva, R., Christopher, B. P., ... & Zayed, H. (2019). Understanding the structure-function relationship of HPRT1 missense mutations in association with Lesch–Nyhan disease and HPRT1-related gout by in silico mutational analysis. Computers in Biology and Medicine, 107, 161-171.

Jeon, R. H., Lee, W. J., Son, Y. B., Bharti, D., Shivakumar, S. B., Lee, S. L., & Rho, G. J. (2019). PPIA, HPRT1, and YWHAZ genes are suitable for normalization of mRNA expression in long-term expanded human mesenchymal stem cells. BioMed Research International, 2019.

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For research use only. Not intended for any clinical use.

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