Mouse Anti-HPRT1 Recombinant Antibody (CBT2530) (V2LY-0625-LY129)

Name: Mouse Anti-HPRT1 Recombinant Antibody (CBT2530)
SKU: V2LY-0625-LY129
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-HPRT1 Recombinant Antibody (CBT2530) (V2LY-0625-LY129) in WB

Mouse Anti-HPRT1 Recombinant Antibody (CBT2530) (V2LY-0625-LY129) in FC

Mouse Anti-HPRT1 Recombinant Antibody (CBT2530) (V2LY-0625-LY129) in IHC

Mouse Anti-HPRT1 Recombinant Antibody (CBT2530) (V2LY-0625-LY129) in ELISA

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2530

Application

WB, IHC, FC

Immunogen

Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

hypoxanthine phosphoribosyltransferase 1

Entrez Gene ID

3251

UniProt ID

P00492

Function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

Biological Process

Adenine metabolic process Source: Ensembl
AMP salvage Source: Ensembl
Central nervous system neuron development Source: Ensembl
Cerebral cortex neuron differentiation Source: Ensembl
Dendrite morphogenesis Source: Ensembl
Dopamine metabolic process Source: Ensembl
GMP catabolic process Source: UniProtKB
GMP salvage Source: MGI
Grooming behavior Source: Ensembl
Guanine salvage Source: UniProtKB
Hypoxanthine metabolic process Source: UniProtKB
Hypoxanthine salvage Source: UniProtKB
IMP metabolic process Source: UniProtKB
IMP salvage Source: GO_Central
Locomotory behavior Source: Ensembl
Lymphocyte proliferation Source: Ensembl
Positive regulation of dopamine metabolic process Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Purine nucleotide biosynthetic process Source: UniProtKB
Purine ribonucleoside salvage Source: UniProtKB
Response to amphetamine Source: Ensembl
Striatum development Source: Ensembl
T cell mediated cytotoxicity Source: Ensembl

Cellular Location

Cytoplasm

Involvement in disease

Lesch-Nyhan syndrome (LNS):
Characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.
Hyperuricemia, HPRT-related (HRH):
An X-linked metabolic disorder characterized by uric acid excess in the blood, renal stones, uric acid nephropathy, and renal obstruction. After puberty, the hyperuricemia may cause gout.

Wu, T., Jiao, Z., Li, Y., Su, X., Yao, F., Peng, J., ... & Yang, A. (2022). HPRT1 promotes chemoresistance in oral squamous cell carcinoma via activating MMP1/PI3K/Akt signaling pathway. Cancers, 14(4), 855.

Li, C., Chen, S., Jia, W., Li, W., Wei, D., Cao, S., ... & Lei, D. (2022). Identify metabolism-related genes IDO1, ALDH2, NCOA2, SLC7A5, SLC3A2, LDHB, and HPRT1 as potential prognostic markers and correlate with immune infiltrates in head and neck squamous cell carcinoma. Frontiers in immunology, 13, 955614.

Lee, S. Y., Choe, Y. H., Han, J. H., Hwang, G., Choi, M. Y., Thakur, G., ... & Hwang, T. S. (2022). HPRT1 Most Suitable Reference Gene for Accurate Normalization of mRNA Expression in Canine Dermal Tissues with Radiation Therapy. Genes, 13(11), 1928.

Ahmadi, M., Eftekhari Kenzerki, M., Akrami, S. M., Pashangzadeh, S., Hajiesmaeili, F., Rahnavard, S., ... & Mousavi, P. (2021). Overexpression of HPRT1 is associated with poor prognosis in head and neck squamous cell carcinoma. FEBS Open Bio, 11(9), 2525-2540.

Yang, Q., Humphreys, S. C., Lade, J. M., & Li, A. P. (2021). Prolonged cultured human hepatocytes as an in vitro experimental system for the evaluation of potency and duration of activity of RNA therapeutics: Demonstration of prolonged duration of gene silencing effects of a GalNAc-conjugated human hypoxanthine phosphoribosyl transferase (HPRT1) siRNA. Biochemical Pharmacology, 189, 114374.

Jiang, J., Piao, X., Hu, S., Gao, J., & Bao, M. (2020). LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/β-catenin signaling pathway. Aging (Albany NY), 12(10), 8820.

Jinnah, H. A. (2020). HPRT1 disorders.

De Mandal, S., Mazumder, T. H., Panda, A. K., Kumar, N. S., & Jin, F. (2020). Analysis of synonymous codon usage patterns of HPRT1 gene across twelve mammalian species. Genomics, 112(1), 304-311.

Agrahari, A. K., Priya, M. K., Kumar, M. P., Tayubi, I. A., Siva, R., Christopher, B. P., ... & Zayed, H. (2019). Understanding the structure-function relationship of HPRT1 missense mutations in association with Lesch–Nyhan disease and HPRT1-related gout by in silico mutational analysis. Computers in Biology and Medicine, 107, 161-171.

Jeon, R. H., Lee, W. J., Son, Y. B., Bharti, D., Shivakumar, S. B., Lee, S. L., & Rho, G. J. (2019). PPIA, HPRT1, and YWHAZ genes are suitable for normalization of mRNA expression in long-term expanded human mesenchymal stem cells. BioMed Research International, 2019.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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