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Mouse Anti-HSD17B12 Recombinant Antibody (4G11) (CBMAB-A4100-LY)

The product is antibody recognizes HSD17B12. The antibody 4G11 immunoassay techniques such as: WB, ELISA.
See all HSD17B12 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
4G11
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
HSD17B12 (NP_057226, 203 a.a. ~ 271 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
hydroxysteroid (17-beta) dehydrogenase 12
Introduction
The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.[supplied by OMIM
Entrez Gene ID
Human51144
Mouse56348
Rat84013
UniProt ID
HumanQ53GQ0
MouseO70503
RatQ6P7R8
Alternative Names
KAR; SDR12C1
Function
Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
Biological Process
Estrogen biosynthetic process Source: UniProtKB-UniPathway
Extracellular matrix organization Source: Ensembl
Fatty acid biosynthetic process Source: UniProtKB-UniPathway
Long-chain fatty-acyl-CoA biosynthetic process Source: Reactome
Positive regulation of cell-substrate adhesion Source: Ensembl
Cellular Location
Endoplasmic reticulum membrane

Ichida, H., Fukami, T., Kudo, T., Mishiro, K., Takano, S., Nakano, M., ... & Nakajima, M. (2023). Identification of HSD17B12 as an enzyme catalyzing drug reduction reactions through investigation of nabumetone metabolism. Archives of Biochemistry and Biophysics, 736, 109536.

Liu, H., Li, G., Sturgis, E. M., Shete, S., Dahlstrom, K. R., Du, M., ... & Wei, Q. (2022). Genetic variants in CYP2B6 and HSD17B12 associated with risk of squamous cell carcinoma of the head and neck. International journal of cancer, 151(4), 553-564.

Juengel, J. L., Mosaad, E. M. O., Mitchell, M. D., Phyn, C. V. C., French, M. C., Meenken, E. D., ... & Meier, S. (2022). Relationships between prostaglandin concentrations, a single nucleotide polymorphism in HSD17B12, and reproductive performance in dairy cows. Journal of Dairy Science, 105(5), 4643-4652.

Wang, Q., Chen, Q., Zhang, Y., Zhang, X., Liu, C., Wang, D., ... & Zhang, F. (2022). HSD17B12 dosage insufficiency induced premature ovarian insufficiency in humans and mice. Clinical and Translational Medicine, 12(2), e737.

Lin, Y., Meng, Y., Zhang, J., Ma, L., Jiang, L., Zhang, Y., ... & Zhu, L. (2020). Functional genetic variant of HSD17B12 in the fatty acid biosynthesis pathway predicts the outcome of colorectal cancer. Journal of Cellular and Molecular Medicine, 24(24), 14160-14170.

Mohamed, B., Mazeaud, C., Baril, M., Poirier, D., Sow, A. A., Chatel-Chaix, L., ... & Lamarre, D. (2020). Very-long-chain fatty acid metabolic capacity of 17-beta-hydroxysteroid dehydrogenase type 12 (HSD17B12) promotes replication of hepatitis C virus and related flaviviruses. Scientific Reports, 10(1), 4040.

Hachim, M. Y., Aljaibeji, H., Hamoudi, R. A., Hachim, I. Y., Elemam, N. M., Mohammed, A. K., ... & Sulaiman, N. (2020). An integrative phenotype–genotype approach using phenotypic characteristics from the UAE national diabetes study identifies HSD17B12 as a candidate gene for obesity and type 2 diabetes. Genes, 11(4), 461.

Dai, W., Liu, H., Xu, X., Ge, J., Luo, S., Zhu, D., ... & Wei, Q. (2019). Genetic variants in ELOVL2 and HSD17B12 predict melanoma‐specific survival. International journal of cancer, 145(10), 2619-2628.

Kemiläinen, H., Huhtinen, K., Auranen, A., Carpén, O., Strauss, L., & Poutanen, M. (2018). The expression of HSD17B12 is associated with COX-2 expression and is increased in high-grade epithelial ovarian cancer. Oncology, 94(4), 233-242.

Yang, Y., Fang, X., Yang, R., Yu, H., Jiang, P., Sun, B., & Zhao, Z. (2018). MiR-152 regulates apoptosis and triglyceride production in MECs via targeting ACAA2 and HSD17B12 genes. Scientific Reports, 8(1), 417.

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For research use only. Not intended for any clinical use.

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