Mouse Anti-HSF2BP Recombinant Antibody (CBFYH-2059) (CBMAB-H0732-FY)

Name: Mouse Anti-HSF2BP Recombinant Antibody (CBFYH-2059)
SKU: CBMAB-H0732-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-2059

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

heat shock transcription factor 2 binding protein

Introduction

HSF2 binding protein (HSF2BP) associates with HSF2. The interaction occurs between the trimerization domain of HSF2 and the amino terminal hydrophilic region of HSF2BP that comprises two leucine zipper motifs. HSF2BP may therefore be involved in modulating HSF2 activation.

Entrez Gene ID

11077

UniProt ID

O75031

Alternative Names

Heat Shock Transcription Factor 2 Binding Protein; Heat Shock Factor 2 Binding Protein

Function

Meiotic recombination factor component of recombination bridges involved in meiotic double-strand break repair. Modulates the localization of recombinases DMC1:RAD51 to meiotic double-strand break (DSB) sites through the interaction with BRCA2 and its recruitment during meiotic recombination (By similarity) (PubMed:31242413).

Indispensable for the DSB repair, homologous synapsis, and crossover formation that are needed for progression past metaphase I, is essential for spermatogenesis and male fertility (By similarity).

Required for proper recombinase recruitment in female meiosis (By similarity).

Inhibits BNC1 transcriptional activity during spermatogenesis, probably by sequestering it in the cytoplasm (By similarity).

May be involved in modulating HSF2 activation in testis (PubMed:9651507).

Biological Process

Double-strand break repair involved in meiotic recombination Source: UniProtKB
Female meiosis I Source: UniProtKB
Male meiosis I Source: UniProtKB
Spermatogenesis Source: UniProtKB
Transcription by RNA polymerase II Source: ProtInc

Cellular Location

Cytoplasm; Chromosome. Localizes on double-strand breaks (DSBs) in mitotic and meiotic chromosomes.

Involvement in disease

Premature ovarian failure 19 (POF19):
A form of premature ovarian failure, an ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. POF19 is an autosomal recessive form characterized by irregular menstrual cycles and cessation of menstruation in the third decade of life.

PTM

Sumoylated by UBE2I in response to MEKK1-mediated stimuli.

Ghouil, R., Miron, S., Sato, K., Ristic, D., van Rossum-Fikkert, S. E., Legrand, P., ... & Zinn-Justin, S. (2023). BRCA2-HSF2BP Oligomeric Ring Disassembly by BRME1 Promotes Homologous Recombination. bioRxiv, 2023-04.

Bi, J., Zhang, J., Ke, M., Wang, T., Wang, M., Liu, W., ... & Wu, R. (2022). HSF2BP protects against acute liver injury by regulating HSF2/HSP70/MAPK signaling in mice. Cell Death & Disease, 13(9), 830.

Li, S., Xu, W., Xu, B., Gao, S., Zhang, Q., Qin, Y., & Guo, T. (2022). Pathogenic variations of homologous recombination gene HSF2BP identified in sporadic patients with premature ovarian insufficiency. Frontiers in Cell and Developmental Biology, 9, 768123.

Ghouil, R., Miron, S., Koornneef, L., Veerman, J., Paul, M. W., Le Du, M. H., ... & Zelensky, A. N. (2021). BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination. Nature Communications, 12(1), 4605.

Huang, Z., Liu, Z., Cheng, X., Han, Z., Li, J., Xia, T., ... & Wei, L. (2021). Prognostic significance of HSF2BP in lung adenocarcinoma. Annals of Translational Medicine, 9(20).

Felipe-Medina, N., Caburet, S., Sánchez-Sáez, F., Condezo, Y. B., De Rooij, D. G., Gómez-h, L., ... & Pendás, A. M. (2020). A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. Elife, 9, e56996.

Sato, K., Brandsma, I., van Rossum-Fikkert, S. E., Verkaik, N., Oostra, A. B., Dorsman, J. C., ... & Zelensky, A. N. (2020). HSF2BP negatively regulates homologous recombination in DNA interstrand crosslink repair. Nucleic Acids Research, 48(5), 2442-2456.

Ghouil, R., Miron, S., Koornneef, L., Veerman, J., Paul, M. W., Le Du, M. H., ... & Zelensky, A. N. (2020). A cryptic BRCA2 repeated motif binds to HSF2BP oligomers with no impact on meiotic recombination. bioRxiv, 2020-12.

Brandsma, I., Sato, K., van Rossum-Fikkert, S. E., van Vliet, N., Sleddens, E., Reuter, M., ... & Zelensky, A. N. (2019). HSF2BP interacts with a conserved domain of BRCA2 and is required for mouse spermatogenesis. Cell reports, 27(13), 3790-3798.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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