Mouse Anti-HYAL3 (AA 1-417) Recombinant Antibody (CBFYH-2564) (CBMAB-H3586-FY)

Name: Mouse Anti-HYAL3 (AA 1-417) Recombinant Antibody (CBFYH-2564)
SKU: CBMAB-H3586-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-2564

Application

ELISA

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-417

More Infomation

Target

Full Name

hyaluronoglucosaminidase 3

Introduction

This gene encodes a member of the hyaluronidase family. Hyaluronidases are endoglycosidase enzymes that degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. The regulated turnover of hyaluronan plays a critical role in many biological processes including cell proliferation, migration and differentiation. The encoded protein may also play an important role in sperm function. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression, and the expression of specific transcript variants may be indicative of tumor status. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and some isoforms may lack hyaluronidase activity. This gene overlaps and is on the same strand as N-acetyltransferase 6 (GCN5-related), and some transcripts of each gene share a portion of the first exon.

Entrez Gene ID

8372

UniProt ID

O43820

Alternative Names

Hyaluronoglucosaminidase 3; Lung Carcinoma Protein 3; EC 3.2.1.35; HYAL-3; LUCA-3; LUCA3; Hyaluronoglucosaminidase-3; Hyaluronidase-3

Function

Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm. Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage. Has no hyaluronidase activity in embryonic fibroblasts in vitro. Has no hyaluronidase activity in granulosa cells in vitro.

Biological Process

Carbohydrate metabolic process Source: InterPro
Cartilage development Source: UniProtKB
Cellular response to interleukin-1 Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Cellular response to UV-B Source: UniProtKB
Hyaluronan catabolic process Source: UniProtKB
Inflammatory response Source: UniProtKB
Negative regulation of ovarian follicle development Source: UniProtKB
Ovarian follicle atresia Source: UniProtKB
Penetration of zona pellucida Source: UniProtKB
Positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
Response to antibiotic Source: UniProtKB
Response to virus Source: UniProtKB

Cellular Location

Secreted; Endoplasmic reticulum; Early endosome; Cell membrane; Acrosome. Mostly present in low-density vesicles. Low levels in higher density vesicles of late endosomes and lysosomes. Localized in punctate cytoplasmic vesicles and in perinuclear structures, but does not colocalize with LAMP1. Localized on the plasma membrane over the acrosome and on the surface of the midpiece of the sperm tail.

PTM

N-glycosylated.

Liu, J. P., Fang, Y. T., Jiang, Y. F., & Lin, H. (2022). HYAL3 as a potential novel marker of BLCA patient prognosis. BMC Genomic Data, 23(1), 1-17.

Aslam, S., & Zhang, Z. (2022). Identification of novel mutation in HYAL3 gene of human oligospermia patients by DNA sequencing.

Krupkova, O., Greutert, H., Boos, N., Lemcke, J., Liebscher, T., & Wuertz-Kozak, K. (2020). Expression and activity of hyaluronidases HYAL-1, HYAL-2 and HYAL-3 in the human intervertebral disc. European Spine Journal, 29, 605-615.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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