Human HYAL3 ELISA Kit (V2LY-0626-LY4702)

Name: Human HYAL3 ELISA Kit
SKU: V2LY-0626-LY4702
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.15 ng/mL

Detection Range

0.3-90 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

46.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

hyaluronoglucosaminidase 3

Function

Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm. Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage. Has no hyaluronidase activity in embryonic fibroblasts in vitro. Has no hyaluronidase activity in granulosa cells in vitro.

Biological Process

Carbohydrate metabolic process Source: InterPro
Cartilage development Source: UniProtKB
Cellular response to interleukin-1 Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Cellular response to UV-B Source: UniProtKB
Hyaluronan catabolic process Source: UniProtKB
Inflammatory response Source: UniProtKB
Negative regulation of ovarian follicle development Source: UniProtKB
Ovarian follicle atresia Source: UniProtKB
Penetration of zona pellucida Source: UniProtKB
Positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
Response to antibiotic Source: UniProtKB
Response to virus Source: UniProtKB

Cellular Location

Secreted; Endoplasmic reticulum; Early endosome; Cell membrane; Acrosome. Mostly present in low-density vesicles. Low levels in higher density vesicles of late endosomes and lysosomes. Localized in punctate cytoplasmic vesicles and in perinuclear structures, but does not colocalize with LAMP1. Localized on the plasma membrane over the acrosome and on the surface of the midpiece of the sperm tail.

PTM

N-glycosylated.

Liu, J. P., Fang, Y. T., Jiang, Y. F., & Lin, H. (2022). HYAL3 as a potential novel marker of BLCA patient prognosis. BMC Genomic Data, 23(1), 1-17.

Aslam, S., & Zhang, Z. (2022). Identification of novel mutation in HYAL3 gene of human oligospermia patients by DNA sequencing.

Krupkova, O., Greutert, H., Boos, N., Lemcke, J., Liebscher, T., & Wuertz-Kozak, K. (2020). Expression and activity of hyaluronidases HYAL-1, HYAL-2 and HYAL-3 in the human intervertebral disc. European Spine Journal, 29, 605-615.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-HYAL3 Recombinant Antibody (3A3) (CAT#: CBMAB-A4177-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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