Mouse Anti-IFNLR1 Recombinant Antibody (
5G12F3) (V2LY-0725-LY489)

Name: Mouse Anti-IFNLR1 Recombinant Antibody (5G12F3)
SKU: V2LY-0725-LY489
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-IFNLR1 Recombinant Antibody (5G12F3) (V2LY-0725-LY489) in IF

Immunofluorescence staining of Hela cells with(V2LY-0725-LY489)at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Mouse Anti-IFNLR1 Recombinant Antibody (5G12F3) (V2LY-0725-LY489) in IF

Immunofluorescence staining of U251 cells with(V2LY-0725-LY489)at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5G12F3

Application

ELISA, IF

Immunogen

Recombinant IFNLR1 protein (aa 21-228).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Interferon Lambda Receptor 1

Entrez Gene ID

163702

UniProt ID

Q8IU57

Function

The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Determines the cell type specificity of the lambda interferon action. Shows a more restricted pattern of expression in the epithelial tissues thereby limiting responses to lambda interferons primarily to epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium.

Biological Process

Cytokine-mediated signaling pathway Source: GO_Central
Defense response to virus Source: UniProtKB
Mucosal immune response Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Positive regulation of cellular respiration Source: ARUK-UCL
Regulation of defense response to virus by host Source: UniProtKB
Response to type III interferon Source: UniProtKB

Cellular Location

Membrane

Topology

Extracellular: 21-228
Helical: 229-249
Cytoplasmic: 250-520

Evans, J. G., Novotny, L. A., & Meissner, E. G. (2023). Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda Signaling. Viruses, 15(3), 632.

Zhang, T. Y., Chen, T., Hu, W. Y., Li, J. C., & Guo, M. Y. (2022). Ammonia induces autophagy via circ‐IFNLR1/miR‐2188‐5p/RNF182 axis in tracheas of chickens. Biofactors, 48(2), 416-427.

Wang, W. Q., Qiu, S. W., Huang, S. S., Wang, G. J., Han, M. Y., Kang, D. Y., ... & Dai, P. (2022). Transcriptome analysis of the early stage ifnlr1-mutant zebrafish indicates the immune response to auditory dysfunction. Gene Expression Patterns, 43, 119229.

Qin, M., Chen, W., Li, Z., Wang, L., Ma, L., Geng, J., ... & Zeng, Y. (2021). Role of IFNLR1 gene in PRRSV infection of PAM cells. Journal of Veterinary Science, 22(3).

Hausmann, L. D., de Almeida, B. S., de Souza, I. R., Drehmer, M. N., Fernandes, B. L., Wilkens, R. S., ... & Muniz, Y. C. N. (2021). Association of TNFRSF1A and IFNLR1 gene polymorphisms with the risk of developing breast cancer and clinical pathologic features. Biochemical Genetics, 59, 1233-1246.

Evans, G., Kappler, C. S., Liu, R., Carten, J. D., Orr, C. M., Stephenson, S., ... & Meissner, E. G. (2021). 49483 Evaluating the Role of IFNLR1 Receptor Dynamics and Plasticity in Regulating Cellular Response to Interferons. Journal of Clinical and Translational Science, 5(s1), 14-15.

Zhang, A. M., Wang, Y., Xia, X., & Song, Y. (2020). Genetic polymorphisms of the IFNLR1 gene correlate with HCV infection and biochemical features of chronic HCV patients in Yunnan, China. Immunological Investigations, 49(4), 453-461.

Ma, N., Zhang, X., Yang, L., Zhou, J., Liu, W., Gao, X., ... & Liu, D. (2018). Role of Functional IFNL 4, IFNLR 1, IFNA, IFNAR 2 Polymorphisms in Hepatitis B virus‐related liver disease in Han Chinese population. Journal of viral hepatitis, 25(3), 306-313.

Gao, X., Yuan, Y. Y., Lin, Q. F., Xu, J. C., Wang, W. Q., Qiao, Y. H., ... & Dai, P. (2018). Mutation of IFNLR1, an interferon lambda receptor 1, is associated with autosomal-dominant non-syndromic hearing loss. Journal of medical genetics, 55(5), 298-306.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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