Mouse Anti-IFT88 Recombinant Antibody (A424) (CBMAB-AP10814LY)

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Basic Information

Host Animal
Mouse
Clone
A424
Application
ELISA, IF, IHC, WB
Immunogen
Fusion protein of IFT88
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
Affinity purity
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Intraflagellar Transport 88
Introduction
This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017]
Entrez Gene ID
Human8100
Mouse21821
Rat305918
UniProt ID
HumanQ13099
MouseQ61371
RatD4ACI9
Alternative Names
Intraflagellar Transport 88
Function
E-AB-27484
Biological Process
Cilium assembly Source: UniProtKB
Inner ear receptor cell stereocilium organization Source: GO_Central
Intraciliary transport Source: GO_Central
Kidney development Source: GO_Central
Non-motile cilium assembly Source: GO_Central
Regulation of autophagosome assembly Source: UniProtKB
Regulation of cilium assembly Source: UniProtKB
Cellular Location
Centriole; Cilium basal body; Centrosome; Cytoplasm; Cilium; Flagellum. Colocalizes with ENTR1 and gamma-tubulin at the basal body of primary cilia. Colocalizes with ENTR1 and pericentrin at the centrosome.

Zhang, Z., Sa, G., Wang, Z., Wei, Z., Zheng, L., Zhang, R., ... & Yang, X. (2022). Piezo1 and IFT88 synergistically regulate mandibular condylar chondrocyte differentiation under cyclic tensile strain. Tissue and Cell, 76, 101781.

Coveney, C. R., Samvelyan, H. J., Miotla‐Zarebska, J., Carnegie, J., Chang, E., Corrin, C. J., ... & Wann, A. K. (2022). Ciliary IFT88 protects coordinated adolescent growth plate ossification from disruptive physiological mechanical forces. Journal of Bone and Mineral Research, 37(6), 1081-1096.

Wang, S., Zhuang, S., & Dong, Z. (2021). IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy. American Journal of Physiology-Renal Physiology, 321(3), F269-F277.

Fujii, R., Hasegawa, S., Maekawa, H., Inoue, T., Yoshioka, K., Uni, R., ... & Inagi, R. (2021). Decreased IFT88 expression with primary cilia shortening causes mitochondrial dysfunction in cisplatin-induced tubular injury. American Journal of Physiology-Renal Physiology, 321(3), F278-F292.

Singh, S., Adam, M., Matkar, P. N., Bugyei-Twum, A., Desjardins, J. F., Chen, H. H., ... & Singh, K. K. (2020). Endothelial-specific loss of IFT88 promotes endothelial-to-mesenchymal transition and exacerbates bleomycin-induced pulmonary fibrosis. Scientific Reports, 10(1), 4466.

Shohayeb, B., Ho, U., Yeap, Y. Y., Parton, R. G., Millard, S. S., Xu, Z., ... & Ng, D. C. (2020). The association of microcephaly protein WDR62 with CPAP/IFT88 is required for cilia formation and neocortical development. Human molecular genetics, 29(2), 248-263.

Coveney, C. R., Collins, I., Mc Fie, M., Chanalaris, A., Yamamoto, K., & Wann, A. K. (2018). Cilia protein IFT88 regulates extracellular protease activity by optimizing LRP-1–mediated endocytosis. The FASEB Journal, 32(12), 6771.

Lee, J., Yi, S., Won, M., Song, Y. S., Yi, H. S., Park, Y. J., ... & Shong, M. (2018). Loss-of-function of IFT88 determines metabolic phenotypes in thyroid cancer. Oncogene, 37(32), 4455-4474.

Takei, R., Katoh, Y., & Nakayama, K. (2018). Robust interaction of IFT70 with IFT52–IFT88 in the IFT-B complex is required for ciliogenesis. Biology open, 7(5), bio033241.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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