Rabbit Anti-IFT88 Recombinant Antibody (CBYJT-1387) (CBMAB-T0409-YJ)

Name: Rabbit Anti-IFT88 Recombinant Antibody (CBYJT-1387)
SKU: CBMAB-T0409-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYJT-1387

Application

WB, FC

Immunogen

Synthetic peptide within Human TTC10 aa 1-100 (N terminal). The exact sequence is proprietary.

Specificity

Human, Rat, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-100

More Infomation

Target

Full Name

Intraflagellar Transport 88

Introduction

IFT88 is a member of the tetratrico peptide repeat (TPR) family. IFT88 is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease.

Entrez Gene ID

Human	8100
Mouse	21821
Rat	305918

UniProt ID

Human	Q13099
Mouse	Q61371
Rat	D4ACI9

Alternative Names

Intraflagellar Transport 88

Function

Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.

Biological Process

Cilium assembly Source: UniProtKB
Inner ear receptor cell stereocilium organization Source: GO_Central
Intraciliary transport Source: GO_Central
Kidney development Source: GO_Central
Non-motile cilium assembly Source: GO_Central
Regulation of autophagosome assembly Source: UniProtKB
Regulation of cilium assembly Source: UniProtKB

Cellular Location

Centriole; Cilium basal body; Centrosome; Cytoplasm; Cilium; Flagellum. Colocalizes with ENTR1 and gamma-tubulin at the basal body of primary cilia. Colocalizes with ENTR1 and pericentrin at the centrosome.

Zhang, Z., Sa, G., Wang, Z., Wei, Z., Zheng, L., Zhang, R., ... & Yang, X. (2022). Piezo1 and IFT88 synergistically regulate mandibular condylar chondrocyte differentiation under cyclic tensile strain. Tissue and Cell, 76, 101781.

Coveney, C. R., Samvelyan, H. J., Miotla‐Zarebska, J., Carnegie, J., Chang, E., Corrin, C. J., ... & Wann, A. K. (2022). Ciliary IFT88 protects coordinated adolescent growth plate ossification from disruptive physiological mechanical forces. Journal of Bone and Mineral Research, 37(6), 1081-1096.

Wang, S., Zhuang, S., & Dong, Z. (2021). IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy. American Journal of Physiology-Renal Physiology, 321(3), F269-F277.

Fujii, R., Hasegawa, S., Maekawa, H., Inoue, T., Yoshioka, K., Uni, R., ... & Inagi, R. (2021). Decreased IFT88 expression with primary cilia shortening causes mitochondrial dysfunction in cisplatin-induced tubular injury. American Journal of Physiology-Renal Physiology, 321(3), F278-F292.

Singh, S., Adam, M., Matkar, P. N., Bugyei-Twum, A., Desjardins, J. F., Chen, H. H., ... & Singh, K. K. (2020). Endothelial-specific loss of IFT88 promotes endothelial-to-mesenchymal transition and exacerbates bleomycin-induced pulmonary fibrosis. Scientific Reports, 10(1), 4466.

Shohayeb, B., Ho, U., Yeap, Y. Y., Parton, R. G., Millard, S. S., Xu, Z., ... & Ng, D. C. (2020). The association of microcephaly protein WDR62 with CPAP/IFT88 is required for cilia formation and neocortical development. Human molecular genetics, 29(2), 248-263.

Coveney, C. R., Collins, I., Mc Fie, M., Chanalaris, A., Yamamoto, K., & Wann, A. K. (2018). Cilia protein IFT88 regulates extracellular protease activity by optimizing LRP-1–mediated endocytosis. The FASEB Journal, 32(12), 6771.

Lee, J., Yi, S., Won, M., Song, Y. S., Yi, H. S., Park, Y. J., ... & Shong, M. (2018). Loss-of-function of IFT88 determines metabolic phenotypes in thyroid cancer. Oncogene, 37(32), 4455-4474.

Takei, R., Katoh, Y., & Nakayama, K. (2018). Robust interaction of IFT70 with IFT52–IFT88 in the IFT-B complex is required for ciliogenesis. Biology open, 7(5), bio033241.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-IFT88 Recombinant Antibody (CF215) (CAT#: CBMAB-FT258LY)

Mouse Anti-IFT88 Recombinant Antibody (A424) (CAT#: CBMAB-AP10814LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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