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Hamster Anti-IL10RA Recombinant Antibody (CBYY-I1396) (CBMAB-I2566-YY)

This product is Hamster antibody that recognizes IL10RA. The antibody CBYY-I1396 can be used for immunoassay techniques such as: WB
See all IL10RA antibodies

Summary

Host Animal
Hamster
Specificity
Mouse
Clone
CBYY-I1396
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
0.2μm filtered solution in PBS
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Interleukin 10 Receptor Subunit Alpha
Introduction
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.
Entrez Gene ID
16154
UniProt ID
Q61727
Alternative Names
Interleukin 10 Receptor Subunit Alpha
Function
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608).

In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952).

These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).
Biological Process
Cytokine-mediated signaling pathway Source: GO_Central
Negative regulation of autophagy Source: UniProtKB
Positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of synapse organization Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Ubiquitin-dependent endocytosis Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm
Involvement in disease
Inflammatory bowel disease 28, autosomal recessive (IBD28):
A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Topology
Extracellular: 22-235
Helical: 236-256
Cytoplasmic: 257-578
PTM
Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.
Ubiquitinated by BTRC; ubiquitination leads to endocytosis and subsequent degradation of IL10RA.

Grk, M., Miskovic, R., Jeremic, I., Basaric, M., Dusanovic Pjevic, M., Jekic, B., ... & Banko, A. (2023). Association of IL10RA, IL10RB, and IL22RA Polymorphisms/Haplotypes with Susceptibility to and Clinical Manifestations of SLE. International Journal of Molecular Sciences, 24(14), 11292.

Mario-Vásquez, J. E., Naranjo-González, C. A., Montiel, J., Zuluaga, L. M., Vásquez, A. M., Tobón-Castaño, A., ... & Segura, C. (2021). Association of variants in IL1B, TLR9, TREM1, IL10RA, and CD3G and Native American ancestry on malaria susceptibility in Colombian populations. Infection, Genetics and Evolution, 87, 104675.

Tang, Z., Zhang, P., Ji, M., Yin, C., Zhao, R., Huang, Z., & Huang, Y. (2021). Characterization of novel and large fragment deletions in exon 1 of the IL10RA gene in Chinese children with very early onset inflammatory bowel diseases. BMC gastroenterology, 21(1), 1-8.

Cheng, S., Li, Z., Zhang, W., Sun, Z., Fan, Z., Luo, J., & Liu, H. (2021). Identification of IL10RA by weighted correlation network analysis and in vitro validation of its association with prognosis of metastatic melanoma. Frontiers in Cell and Developmental Biology, 8, 630790.

Prokoph, N., Probst, N. A., Lee, L. C., Monahan, J. M., Matthews, J. D., Liang, H. C., ... & Turner, S. D. (2020). IL10RA modulates crizotinib sensitivity in NPM1-ALK+ anaplastic large cell lymphoma. Blood, The Journal of the American Society of Hematology, 136(14), 1657-1669.

Xue, A. J., Miao, S. J., Sun, H., Qiu, X. X., Wang, S. N., Wang, L., ... & Huang, Y. (2020). Intestinal dysbiosis in pediatric Crohn's disease patients with IL10RA mutations. World journal of gastroenterology, 26(22), 3098.

Al-Abbasi, F. A., Mohammed, K., Sadath, S., Banaganapalli, B., Nasser, K., & Shaik, N. A. (2018). Computational protein phenotype characterization of IL10RA mutations causative to early onset inflammatory bowel disease (IBD). Frontiers in Genetics, 9, 146.

Peng, K., Qian, X., Huang, Z., Lu, J., Wang, Y., Zhou, Y., ... & Huang, Y. (2018). Umbilical cord blood transplantation corrects very early-onset inflammatory bowel disease in Chinese patients with IL10RA-associated immune deficiency. Inflammatory Bowel Diseases, 24(7), 1416-1427.

Zadka, Ł., Kulus, M. J., Kurnol, K., Piotrowska, A., Glatzel-Plucińska, N., Jurek, T., ... & Dzięgiel, P. (2018). The expression of IL10RA in colorectal cancer and its correlation with the proliferation index and the clinical stage of the disease. Cytokine, 110, 116-125.

Jung, E. S., Petersen, B. S., Mayr, G., Cheon, J. H., Kang, Y., Lee, S. J., ... & Koh, H. (2018). Compound heterozygous mutations in IL10RA combined with a complement factor properdin mutation in infantile-onset inflammatory bowel disease. European Journal of Gastroenterology & Hepatology, 30(12), 1491-1496.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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