Rat Anti-IL15RA Recombinant Antibody (888220) (CBMAB-I1740-YY)

Name: Rat Anti-IL15RA Recombinant Antibody (888220)
SKU: CBMAB-I1740-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

888220

Application

FC, CyTOF

Specificity

Mouse

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

0.2 μm filtered solution in with Trehalose

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

interleukin 15 receptor subunit alpha

Introduction

This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.

Entrez Gene ID

16169

UniProt ID

Q60819

Alternative Names

Interleukin 15 Receptor Subunit Alpha

Function

High-affinity receptor for interleukin-15 (PubMed:8530383).

Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity).

In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed:15123770).

In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed:15123770).

Expression of different isoforms may alter or interfere with signal transduction (PubMed:10480910).

Isoform 5&6&7&8:
Does not bind IL15.

Biological Process

Interleukin-15-mediated signaling pathway Source: UniProtKB
Positive regulation of natural killer cell differentiation Source: Ensembl
Positive regulation of phagocytosis Source: UniProtKB

Cellular Location

Nucleus membrane; Membrane; Cell surface. Mainly found associated with the nuclear membrane.
Isoform 5: Golgi apparatus membrane; Endoplasmic reticulum membrane; Cytoplasmic vesicle membrane; Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
Isoform 6: Golgi apparatus membrane; Endoplasmic reticulum membrane; Cytoplasmic vesicle membrane; Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
Isoform 7: Golgi apparatus membrane; Endoplasmic reticulum membrane; Cytoplasmic vesicle membrane; Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
Isoform 8: Golgi apparatus membrane; Endoplasmic reticulum membrane; Cytoplasmic vesicle membrane; Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
Soluble interleukin-15 receptor subunit alpha: Extracellular space

Topology

Extracellular: 31-205
Helical: 206-228
Cytoplasmic: 229-267

PTM

N-glycosylated and O-glycosylated.
A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor (PubMed:15265897). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor (PubMed:15265897).

Liu, Z., Zou, W., Xu, Y., & Wu, P. (2023). Clinical characterization and immunosuppressive regulation of IL15RA in Kidney renal papillary cell carcinoma through 324 samples.

Fu, S., Kundranda, M. N., Rahimian, S., Shen, Y., Tan, Q., Zhao, R., ... & Liang, T. (2023). The initial report of phase I trial of VG2025, a non-attenuated HSV-1 oncolytic virus expressing IL-12 and IL-15/RA payloads, in patients with advanced solid tumors.

Bellón, T., González-Valle, O., Sendagorta, E., Lerma, V., Martínez del Río, J., Martínez, C., ... & de Abajo, F. (2022). IL-15/IL-15Rα in SJS/TEN: Relevant Expression of IL15 and IL15RA in Affected Skin. Biomedicines, 10(8), 1868.

De Mattia, E., Polesel, J., Roncato, R., Labriet, A., Bignucolo, A., Gagno, S., ... & Toffoli, G. (2021). IL15RA and SMAD3 genetic variants predict overall survival in metastatic colorectal cancer patients treated with folfiri therapy: A new paradigm. Cancers, 13(7), 1705.

Warner, S. C., Nair, A., Marpadga, R., Chubinskaya, S., Doherty, M., Valdes, A. M., & Scanzello, C. R. (2020). IL-15 and IL15RA in osteoarthritis: association with symptoms and protease production, but not structural severity. Frontiers in immunology, 11, 1385.

Yang, H., Zhou, L., Chen, J., Su, J., Shen, W., Liu, B., ... & Qian, J. (2019). A four‑gene signature for prognosis in breast cancer patients with hypermethylated IL15RA. Oncology Letters, 17(5), 4245-4254.

Loro, E., Bisetto, S., & Khurana, T. S. (2018). Mitochondrial ultrastructural adaptations in fast muscles of mice lacking IL15RA. Journal of Cell Science, 131(21), jcs218313.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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