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Mouse Anti-IRAK3 Recombinant Antibody (1C8) (CBMAB-A4517-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1C8
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
IRAK3 (AAH57800, 497 a.a. ~ 596 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Interleukin 1 Receptor Associated Kinase 3
Entrez Gene ID
11213
UniProt ID
Q9Y616
Alternative Names
ASRT5; FLJ13601; IRAK-M; IRAKM
Function
Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:10383454, PubMed:29686383).
Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity).
Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383).
Biological Process
Cytokine-mediated signaling pathwayISS:UniProtKB
Interleukin-1-mediated signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
MyD88-dependent toll-like receptor signaling pathwayManual Assertion Based On ExperimentTAS:BHF-UCL
Negative regulation of cytokine-mediated signaling pathway1 PublicationIC:BHF-UCL
Negative regulation of innate immune responseBy SimilarityISS:BHF-UCL
Negative regulation of interleukin-12 productionManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of interleukin-6 productionManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of macrophage cytokine productionBy SimilarityISS:BHF-UCL
Negative regulation of MAP kinase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of protein catabolic processManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of protein-containing complex disassemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of toll-like receptor signaling pathwayBy SimilarityISS:BHF-UCL
Negative regulation of tumor necrosis factor productionManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of cytokine productionIEA:Ensembl
Positive regulation of macrophage tolerance inductionBy SimilarityISS:BHF-UCL
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIMP:BHF-UCL
Protein autophosphorylationIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of protein-containing complex disassemblyISS:UniProtKB
Response to exogenous dsRNABy SimilarityISS:BHF-UCL
Response to interleukin-1Manual Assertion Based On ExperimentIMP:BHF-UCL
Response to lipopolysaccharideBy SimilarityISS:BHF-UCL
Response to peptidoglycanBy SimilarityISS:BHF-UCL
Response to virus1 PublicationIC:BHF-UCL
Cellular Location
Cytoplasm; Nucleus. In dendritic cells, translocates into the nucleus upon IL33 stimulation.
Involvement in disease
Asthma-related traits 5 (ASRT5):
Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy and atopic dermatitis.
More Infomation

Nguyen, T. H., Turek, I., Meehan-Andrews, T., Zacharias, A., & Irving, H. R. (2022). A systematic review and meta-analyses of interleukin-1 receptor associated kinase 3 (IRAK3) action on inflammation in in vivo models for the study of sepsis. Plos one, 17(2), e0263968.

Nguyen, T. H., Axell, A., Turek, I., Wright, B., Meehan-Andrews, T., & Irving, H. R. (2022). Modulation of inflammatory cytokine production in human monocytes by cGMP and IRAK3. International Journal of Molecular Sciences, 23(5), 2552.

Lange, S. M., Nelen, M. I., Cohen, P., & Kulathu, Y. (2021). Dimeric structure of the pseudokinase IRAK3 suggests an allosteric mechanism for negative regulation. Structure, 29(3), 238-251.

Yu, J., Qin, Y., & Zhou, N. (2021). Knockdown of Circ_SLC39A8 protects against the progression of osteoarthritis by regulating miR-591/IRAK3 axis. Journal of orthopaedic surgery and research, 16(1), 1-10.

Leng, W., Fan, D., Ren, Z., & Li, Q. (2021). Identification of upregulated NF-κB inhibitor alpha and IRAK3 targeting lncRNA following intracranial aneurysm rupture-induced subarachnoid hemorrhage. BMC neurology, 21(1), 1-11.

Nguyen, T. H., Turek, I., Meehan-Andrews, T., Zacharias, A., & Irving, H. (2020). Analysis of interleukin-1 receptor associated kinase-3 (IRAK3) function in modulating expression of inflammatory markers in cell culture models: A systematic review and meta-analysis. Plos one, 15(12), e0244570.

Degorce, S. L., Tavana, O., Banks, E., Crafter, C., Gingipalli, L., Kouvchinov, D., ... & Edmondson, S. D. (2020). Discovery of proteolysis-targeting chimera molecules that selectively degrade the IRAK3 pseudokinase. Journal of Medicinal Chemistry, 63(18), 10460-10473.

Freihat, L. A., Wheeler, J. I., Wong, A., Turek, I., Manallack, D. T., & Irving, H. R. (2019). IRAK3 modulates downstream innate immune signalling through its guanylate cyclase activity. Scientific reports, 9(1), 15468.

Sode, J., Vogel, U., Bank, S., Andersen, P. S., Hetland, M. L., Locht, H., ... & Andersen, V. (2018). Confirmation of an IRAK3 polymorphism as a genetic marker predicting response to anti-TNF treatment in rheumatoid arthritis. The pharmacogenomics journal, 18(1), 81-86.

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For research use only. Not intended for any clinical use.

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