Mouse Anti-KDM3A Recombinant Antibody (CBT2987) (V2LY-0625-LY2499)

Name: Mouse Anti-KDM3A Recombinant Antibody (CBT2987)
SKU: V2LY-0625-LY2499
Rating: 5 (1 reviews)

See all KDM3A antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-KDM3A Recombinant Antibody (CBT2987) (V2LY-0625-LY2499) in WB

Mouse Anti-KDM3A Recombinant Antibody (CBT2987) (V2LY-0625-LY2499) in IF

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2987

Application

WB, ICC

Immunogen

Purified recombinant fragment of human KDM3A expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Lysine Demethylase 3A

Entrez Gene ID

55818

UniProt ID

Q9Y4C1

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Biological Process

Androgen receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to leukemia inhibitory factorIEA:Ensembl
Chromatin organizationIEA:UniProtKB-KW
Formaldehyde biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 dimethylationIEA:Ensembl
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of histone H3-K9 methylationIEA:Ensembl
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of stem cell differentiationIEA:Ensembl
Regulation of stem cell population maintenanceIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Spermatid nucleus elongationIEA:Ensembl

Cellular Location

Cytoplasm; Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).

Zhang, W., Liu, R., Zhang, L., Wang, C., Dong, Z., Feng, J., ... & Wei, Q. (2022). Downregulation of miR-335 exhibited an oncogenic effect via promoting KDM3A/YAP1 networks in clear cell renal cell carcinoma. Cancer Gene Therapy, 29(5), 573-584.

Hou, X., Li, Q., Yang, L., Yang, Z., He, J., Li, Q., & Li, D. (2021). KDM1A and KDM3A promote tumor growth by upregulating cell cycle-associated genes in pancreatic cancer. Experimental Biology and Medicine, 246(17), 1869-1883.

Zhu, Z., Wu, X., Li, Q., Zhang, J., Yu, S., Shen, Q., ... & Hua, J. (2021). Histone demethylase complexes KDM3A and KDM3B cooperate with OCT4/SOX2 to define a pluripotency gene regulatory network. The FASEB Journal, 35(6), e21664.

Yoo, J., Jeon, Y. H., Cho, H. Y., Lee, S. W., Kim, G. W., Lee, D. H., & Kwon, S. H. (2020). Advances in histone demethylase KDM3A as a cancer therapeutic target. Cancers, 12(5), 1098.

Macedo-Silva, C., Miranda-Gonçalves, V., Lameirinhas, A., Lencart, J., Pereira, A., Lobo, J., ... & Jerónimo, C. (2020). JmjC-KDMs KDM3A and KDM6B modulate radioresistance under hypoxic conditions in esophageal squamous cell carcinoma. Cell Death & Disease, 11(12), 1068.

Wang, H. Y., Long, Q. Y., Tang, S. B., Xiao, Q., Gao, C., Zhao, Q. Y., ... & Wu, M. (2019). Histone demethylase KDM3A is required for enhancer activation of hippo target genes in colorectal cancer. Nucleic Acids Research, 47(5), 2349-2364.

Qian, X., Li, X., Shi, Z., Bai, X., Xia, Y., Zheng, Y., ... & Lu, Z. (2019). KDM3A senses oxygen availability to regulate PGC-1α-mediated mitochondrial biogenesis. Molecular cell, 76(6), 885-895.

Liu, J., Liang, T., & Zhangsun, W. (2019). KDM3A is associated with tumor metastasis and modulates colorectal cancer cell migration and invasion. International journal of biological macromolecules, 126, 318-325.

Jones, D., Wilson, L., Thomas, H., Gaughan, L., & Wade, M. A. (2019). The histone demethylase enzymes KDM3A and KDM4B co-operatively regulate chromatin transactions of the estrogen receptor in breast cancer. Cancers, 11(8), 1122.

Kim, H., Kim, D., Choi, S. A., Kim, C. R., Oh, S. K., Pyo, K. E., ... & Baek, S. H. (2018). KDM3A histone demethylase functions as an essential factor for activation of JAK2− STAT3 signaling pathway. Proceedings of the National Academy of Sciences, 115(46), 11766-11771.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-KDM3A Recombinant Antibody (CBT2987)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KDM3A Recombinant Antibody (20) (CAT#: CBMAB-K0678-LY)

Mouse Anti-KDM3A Recombinant Antibody (2-00E-01) (CAT#: CBMAB-K0680-LY)

Mouse Anti-KDM3A Recombinant Antibody (CBT3449) (CAT#: V2LY-0625-LY609)

Mouse Anti-KDM3A Recombinant Antibody (13B438) (CAT#: CBMAB-K0675-LY)

Mouse Anti-KDM3A Recombinant Antibody (CBT799) (CAT#: V2LY-0625-LY3264)

Mouse Anti-KDM3A Recombinant Antibody (1E12) (CAT#: CBMAB-0094-WJ)

Mouse Anti-KDM3A Recombinant Antibody (CBYJL-031) (CAT#: CBMAB-L0061-YJ)

Mouse Anti-KDM3A Recombinant Antibody (CBT2766) (CAT#: V2LY-0625-LY610)

Mouse Anti-KDM3A Recombinant Antibody (CBT800) (CAT#: V2LY-0625-LY3263)

Mouse Anti-KDM3A Recombinant Antibody (LEP-02) (CAT#: CBMAB-K0682-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry