Mouse Anti-KDM6B Recombinant Antibody (3J7) (CBMAB-K0716-LY)

Name: Mouse Anti-KDM6B Recombinant Antibody (3J7)
SKU: CBMAB-K0716-LY
Rating: 5 (1 reviews)

See all KDM6B antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3J7

Application

WB, IP, FC, ICC/IF

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

KDM6B

Introduction

The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]

Entrez Gene ID

23135

UniProt ID

O15054

Alternative Names

Lysine Demethylase 6B; Jumonji Domain Containing 3; Histone Lysine Demethylase; Jumonji Domain-Containing Protein 3; Lysine (K)-Specific Demethylase 6B; JmjC Domain-Containing Protein 3; JMJD3;

Function

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914).
Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914).
Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).
Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402).
Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).

Biological Process

Cardiac muscle cell differentiationBy SimilarityISS:BHF-UCL
Cell fate commitmentIEA:Ensembl
Cellular response to hydrogen peroxideIEA:Ensembl
Chromatin remodelingISS:UniProtKB
Endothelial cell differentiationBy SimilarityISS:BHF-UCL
Heart developmentManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-K27 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Inflammatory responseIEA:UniProtKB-KW
Mesodermal cell differentiationBy SimilarityISS:BHF-UCL
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of transcription by RNA polymerase IIBy SimilarityISS:BHF-UCL
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Involvement in disease

Neurodevelopmental disorder with coarse facies and mild distal skeletal abnormalities (NEDCFSA):
An autosomal dominant disorder characterized by global developmental delay, variable intellectual disability, poor language acquisition, and dysmorphic facial features including a prominent nasal bridge and coarse features. Some patients manifest autism spectrum disorder. Musculoskeletal features may be present and include widened and thickened hands and fingers, joint hypermobility, clinodactyly of the fifth fingers, and toe syndactyly.

Jiang, Y., Li, F., Gao, B., Ma, M., Chen, M., Wu, Y., ... & Shen, H. (2021). KDM6B-mediated histone demethylation of LDHA promotes lung metastasis of osteosarcoma. Theranostics, 11(8), 3868.

Lagunas-Rangel, F. A. (2021). KDM6B (JMJD3) and its dual role in cancer. Biochimie, 184, 63-71.

Cao, Z., Shi, X., Tian, F., Fang, Y., Wu, J. B., Mrdenovic, S., ... & Wang, F. (2021). KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer. Cell Death & Disease, 12(1), 2.

Weber, C., Zhou, Y., Lee, J. G., Looger, L. L., Qian, G., Ge, C., & Capel, B. (2020). Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science, 368(6488), 303-306.

Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller‐Ramey, J., ... & Louie, R. J. (2019). Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. American Journal of Medical Genetics Part A, 179(7), 1276-1286.

Mallaney, C., Ostrander, E. L., Celik, H., Kramer, A. C., Martens, A., Kothari, A., ... & Challen, G. A. (2019). Kdm6b regulates context-dependent hematopoietic stem cell self-renewal and leukemogenesis. Leukemia, 33(10), 2506-2521.

Wijayatunge, R., Liu, F., Shpargel, K. B., Wayne, N. J., Chan, U., Boua, J. V., ... & West, A. E. (2018). The histone demethylase Kdm6b regulates a mature gene expression program in differentiating cerebellar granule neurons. Molecular and Cellular Neuroscience, 87, 4-17.

Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., ... & Capel, B. (2018). The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science, 360(6389), 645-648.

Wei, Y., Zheng, H., Bao, N., Jiang, S., Bueso-Ramos, C. E., Khoury, J., ... & Garcia-Manero, G. (2018). KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in mice. Blood advances, 2(19), 2491-2504.

Neele, A. E., Gijbels, M. J., van der Velden, S., Hoeksema, M. A., Boshuizen, M. C., Prange, K. H., ... & de Winther, M. P. (2018). Myeloid Kdm6b deficiency results in advanced atherosclerosis. Atherosclerosis, 275, 156-165.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-KDM6B Recombinant Antibody (3J7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry