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Mouse Anti-KDM6B Recombinant Antibody (3J7) (CBMAB-K0716-LY)

This product is antibody recognizes KDM6B. The antibody 3J7 immunoassay techniques such as: WB, IP, FC, ICC/IF.
See all KDM6B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3J7
Antibody Isotype
IgG
Application
WB, IP, FC, ICC/IF

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
KDM6B
Introduction
The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]
Entrez Gene ID
23135
UniProt ID
O15054
Alternative Names
Lysine Demethylase 6B; Jumonji Domain Containing 3; Histone Lysine Demethylase; Jumonji Domain-Containing Protein 3; Lysine (K)-Specific Demethylase 6B; JmjC Domain-Containing Protein 3; JMJD3;
Function
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914).
Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914).
Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).
Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402).
Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).
Biological Process
Cardiac muscle cell differentiationBy SimilarityISS:BHF-UCL
Cell fate commitmentIEA:Ensembl
Cellular response to hydrogen peroxideIEA:Ensembl
Chromatin remodelingISS:UniProtKB
Endothelial cell differentiationBy SimilarityISS:BHF-UCL
Heart developmentManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-K27 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Inflammatory responseIEA:UniProtKB-KW
Mesodermal cell differentiationBy SimilarityISS:BHF-UCL
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of transcription by RNA polymerase IIBy SimilarityISS:BHF-UCL
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Involvement in disease
Neurodevelopmental disorder with coarse facies and mild distal skeletal abnormalities (NEDCFSA):
An autosomal dominant disorder characterized by global developmental delay, variable intellectual disability, poor language acquisition, and dysmorphic facial features including a prominent nasal bridge and coarse features. Some patients manifest autism spectrum disorder. Musculoskeletal features may be present and include widened and thickened hands and fingers, joint hypermobility, clinodactyly of the fifth fingers, and toe syndactyly.

Jiang, Y., Li, F., Gao, B., Ma, M., Chen, M., Wu, Y., ... & Shen, H. (2021). KDM6B-mediated histone demethylation of LDHA promotes lung metastasis of osteosarcoma. Theranostics, 11(8), 3868.

Lagunas-Rangel, F. A. (2021). KDM6B (JMJD3) and its dual role in cancer. Biochimie, 184, 63-71.

Cao, Z., Shi, X., Tian, F., Fang, Y., Wu, J. B., Mrdenovic, S., ... & Wang, F. (2021). KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer. Cell Death & Disease, 12(1), 2.

Weber, C., Zhou, Y., Lee, J. G., Looger, L. L., Qian, G., Ge, C., & Capel, B. (2020). Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science, 368(6488), 303-306.

Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller‐Ramey, J., ... & Louie, R. J. (2019). Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. American Journal of Medical Genetics Part A, 179(7), 1276-1286.

Mallaney, C., Ostrander, E. L., Celik, H., Kramer, A. C., Martens, A., Kothari, A., ... & Challen, G. A. (2019). Kdm6b regulates context-dependent hematopoietic stem cell self-renewal and leukemogenesis. Leukemia, 33(10), 2506-2521.

Wijayatunge, R., Liu, F., Shpargel, K. B., Wayne, N. J., Chan, U., Boua, J. V., ... & West, A. E. (2018). The histone demethylase Kdm6b regulates a mature gene expression program in differentiating cerebellar granule neurons. Molecular and Cellular Neuroscience, 87, 4-17.

Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., ... & Capel, B. (2018). The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science, 360(6389), 645-648.

Wei, Y., Zheng, H., Bao, N., Jiang, S., Bueso-Ramos, C. E., Khoury, J., ... & Garcia-Manero, G. (2018). KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in mice. Blood advances, 2(19), 2491-2504.

Neele, A. E., Gijbels, M. J., van der Velden, S., Hoeksema, M. A., Boshuizen, M. C., Prange, K. H., ... & de Winther, M. P. (2018). Myeloid Kdm6b deficiency results in advanced atherosclerosis. Atherosclerosis, 275, 156-165.

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For research use only. Not intended for any clinical use.

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