Human Recombinant KDM6B protein, GST Tag (V2LY-0526-LY5147)

Name: Human Recombinant KDM6B protein, GST Tag
SKU: V2LY-0526-LY5147
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant KDM6B protein, GST Tag consist of Amino Acid: 1043-1682 and predicts a molecular mass of 99 kDa.

Molecule Mass

99 kDa

Sequence

Amino Acid: 1043-1682

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

KDM6B

Function

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914).
Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914).
Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).
Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402).
Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).

Biological Process

Cardiac muscle cell differentiationBy SimilarityISS:BHF-UCL
Cell fate commitmentIEA:Ensembl
Cellular response to hydrogen peroxideIEA:Ensembl
Chromatin remodelingISS:UniProtKB
Endothelial cell differentiationBy SimilarityISS:BHF-UCL
Heart developmentManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-K27 demethylationManual Assertion Based On ExperimentIMP:UniProtKB
Inflammatory responseIEA:UniProtKB-KW
Mesodermal cell differentiationBy SimilarityISS:BHF-UCL
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of transcription by RNA polymerase IIBy SimilarityISS:BHF-UCL
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Involvement in disease

Neurodevelopmental disorder with coarse facies and mild distal skeletal abnormalities (NEDCFSA):
An autosomal dominant disorder characterized by global developmental delay, variable intellectual disability, poor language acquisition, and dysmorphic facial features including a prominent nasal bridge and coarse features. Some patients manifest autism spectrum disorder. Musculoskeletal features may be present and include widened and thickened hands and fingers, joint hypermobility, clinodactyly of the fifth fingers, and toe syndactyly.

Jiang, Y., Li, F., Gao, B., Ma, M., Chen, M., Wu, Y., ... & Shen, H. (2021). KDM6B-mediated histone demethylation of LDHA promotes lung metastasis of osteosarcoma. Theranostics, 11(8), 3868.

Lagunas-Rangel, F. A. (2021). KDM6B (JMJD3) and its dual role in cancer. Biochimie, 184, 63-71.

Cao, Z., Shi, X., Tian, F., Fang, Y., Wu, J. B., Mrdenovic, S., ... & Wang, F. (2021). KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer. Cell Death & Disease, 12(1), 2.

Weber, C., Zhou, Y., Lee, J. G., Looger, L. L., Qian, G., Ge, C., & Capel, B. (2020). Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b. Science, 368(6488), 303-306.

Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller‐Ramey, J., ... & Louie, R. J. (2019). Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. American Journal of Medical Genetics Part A, 179(7), 1276-1286.

Mallaney, C., Ostrander, E. L., Celik, H., Kramer, A. C., Martens, A., Kothari, A., ... & Challen, G. A. (2019). Kdm6b regulates context-dependent hematopoietic stem cell self-renewal and leukemogenesis. Leukemia, 33(10), 2506-2521.

Wijayatunge, R., Liu, F., Shpargel, K. B., Wayne, N. J., Chan, U., Boua, J. V., ... & West, A. E. (2018). The histone demethylase Kdm6b regulates a mature gene expression program in differentiating cerebellar granule neurons. Molecular and Cellular Neuroscience, 87, 4-17.

Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., ... & Capel, B. (2018). The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science, 360(6389), 645-648.

Wei, Y., Zheng, H., Bao, N., Jiang, S., Bueso-Ramos, C. E., Khoury, J., ... & Garcia-Manero, G. (2018). KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in mice. Blood advances, 2(19), 2491-2504.

Neele, A. E., Gijbels, M. J., van der Velden, S., Hoeksema, M. A., Boshuizen, M. C., Prange, K. H., ... & de Winther, M. P. (2018). Myeloid Kdm6b deficiency results in advanced atherosclerosis. Atherosclerosis, 275, 156-165.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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