Mouse Anti-KIF25 Recombinant Antibody (2B4) (CBMAB-K0866-LY)

Name: Mouse Anti-KIF25 Recombinant Antibody (2B4)
SKU: CBMAB-K0866-LY
Rating: 5 (1 reviews)

See all KIF25 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2B4

Application

IF, WB

Immunogen

Full length human recombinant protein of human KIF25(NP_005346) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kinesin Family Member 25

Introduction

The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation. [provided by RefSeq, Jul 2008]

Entrez Gene ID

3834

UniProt ID

Q9UIL4

Alternative Names

Kinesin Family Member 25; Kinesin-Like Protein 3; Kinesin-Like 3; KNSL3; Kinesin-Like Protein KIF25;

Function

Minus-end microtubule-dependent motor protein (By similarity).
Acts as a negative regulator of centrosome separation required to prevent premature centrosome separation during interphase (PubMed:28263957).
Required to maintain a centered nucleus to ensure that the spindle is stably oriented at the onset of mitosis (PubMed:28263957).
May also act as a negative regulator of amino acid starvation-induced autophagy (PubMed:22354037).

Biological Process

Establishment of spindle orientationManual Assertion Based On ExperimentIDA:UniProtKB
Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Mitotic sister chromatid segregationManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of autophagyManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of mitotic centrosome separationManual Assertion Based On ExperimentIDA:UniProtKB
Nucleus localizationManual Assertion Based On ExperimentIDA:UniProtKB
Organelle organizationManual Assertion Based On ExperimentTAS:ProtInc
Protein homotetramerizationISS:UniProtKB

Cellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-KIF25 Recombinant Antibody (2B4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KIF25 Recombinant Antibody (1H10) (CAT#: CBMAB-K0865-LY)

Mouse Anti-KIF25 Recombinant Antibody (2D6) (CAT#: CBMAB-K0868-LY)

Mouse Anti-KIF25 Recombinant Antibody (2C9) (CAT#: CBMAB-K0867-LY)

Mouse Anti-KIF25 Recombinant Antibody (1A6) (CAT#: CBMAB-K0862-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry