Rabbit Anti-KIFAP3 Recombinant Antibody (
28E9) (V2LY-0725-LY1420)

Name: Rabbit Anti-KIFAP3 Recombinant Antibody (28E9)
SKU: V2LY-0725-LY1420
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-KIFAP3 Recombinant Antibody (28E9) (V2LY-0725-LY1420) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: KIFAP3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 92, 87, 83 kDa
Observed band size: 72-95 kDa

Rabbit Anti-KIFAP3 Recombinant Antibody (28E9) (V2LY-0725-LY1420) in IHC

IHC image of V2LY-0725-LY1420 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-KIFAP3 Recombinant Antibody (28E9) (V2LY-0725-LY1420) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY1420 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 552-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

28E9

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human KIFAP3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kinesin Associated Protein 3

Entrez Gene ID

22920

UniProt ID

Q92845

Function

Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity).

Biological Process

Cilium organizationManual Assertion Based On ExperimentIBA:GO_Central
Microtubule-based movementManual Assertion Based On ExperimentIBA:GO_Central
Microtubule-based processISS:UniProtKB
Negative regulation of apoptotic processIEA:Ensembl
Negative regulation of cell population proliferationIEA:Ensembl
Plus-end-directed vesicle transport along microtubuleManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of calcium-dependent cell-cell adhesionIEA:Ensembl
Protein localizationIEA:Ensembl
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Axoneme; Centrosome; Ciliary basal body; Ciliary tip; Ciliary transition zone; Cilium; Condensed nuclear chromosome; Cytosol; Endoplasmic reticulum; Golgi apparatus; Microtubule cytoskeleton

PTM

Phosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1.

Onodera, S., Morita, N., Nakamura, Y., Takahashi, S., Hashimoto, K., Nomura, T., ... & Azuma, T. (2021). Novel alterations in IFT172 and KIFAP3 may induce basal cell carcinoma. Orphanet journal of rare diseases, 16(1), 1-7.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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