Mouse Anti-KLF1 Recombinant Antibody (7B2) (CBMAB-K1039-LY)

Name: Mouse Anti-KLF1 Recombinant Antibody (7B2)
SKU: CBMAB-K1039-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7B2

Application

CIP, IP, WB

Immunogen

This EKLF antibody was raised against a full-length recombinant protein to mouse EKLF (Kruppel-like factor 1 (erythroid))

Specificity

Mouse

Antibody Isotype

IgG3

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kruppel like factor 15

Introduction

This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009]

Entrez Gene ID

16596

UniProt ID

P46099

Alternative Names

Kruppel Like Factor 1; Erythroid Krueppel-Like Transcription Factor; Erythroid Kruppel-Like Factor; EKLF; Erythroid-Specific Transcription Factor EKLF; Kruppel-Like Factor 1 (Erythroid); Krueppel-Like Factor 1;

Function

ranscription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.

Biological Process

Cellular response to peptideIEA:Ensembl
Erythrocyte differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Maternal process involved in female pregnancyIEA:Ensembl
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus. Colocalizes with SUMO1 in nuclear speckles.

Involvement in disease

Anemia, congenital dyserythropoietic, 4 (CDAN4):
A blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.

PTM

Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (By similarity).
Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex (By similarity).
Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity (By similarity).

Suzuki, H., Ogawa, T., Fujita, S., Sone, R., & Kawahara, A. (2023). Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis. Scientific Reports, 13(1), 12279.

Hermeling, J. C., Herholz, M., Baumann, L., Cores, E. C., Zečić, A., Hoppe, T., ... & Trifunovic, A. (2022). Mitochondria-originated redox signalling regulates KLF-1 to promote longevity in Caenorhabditis elegans. Redox Biology, 58, 102533.

Kulczynska-Figurny, K., Bieker, J. J., & Siatecka, M. (2020). Severe anemia caused by dominant mutations in Krüppel-like factor 1 (KLF1). Mutation Research/Reviews in Mutation Research, 786, 108336.

Kulczynska, K., Bieker, J. J., & Siatecka, M. (2019). A Krüppel-like factor 1 (KLF1) mutation associated with severe congenital dyserythropoietic anemia alters its DNA-binding specificity. Molecular and cellular biology.

Herholz, M., Cepeda, E., Baumann, L., Kukat, A., Hermeling, J., Maciej, S., ... & Trifunovic, A. (2019). KLF-1 orchestrates a xenobiotic detoxification program essential for longevity of mitochondrial mutants. Nature Communications, 10(1), 3323.

Teruya, S., Okamura, T., Komai, T., Inoue, M., Iwasaki, Y., Sumitomo, S., ... & Fujio, K. (2018). Egr2-independent, Klf1-mediated induction of PD-L1 in CD4+ T cells. Scientific Reports, 8(1), 7021.

Gillinder, K. R., Magor, G., Bell, C., Ilsley, M. D., Huang, S., & Perkins, A. (2018). KLF1 acts as a pioneer transcription factor to open chromatin and facilitate recruitment of GATA1. Blood, 132, 501.

Keller, J., Vege, S., Horn, T., Keller, M. A., Leger, R. M., Aeschlimann, J., ... & Westhoff, C. M. (2018). Novel mutations in KLF1 encoding the In (Lu) phenotype reflect a diversity of clinical presentations. Transfusion, 58(1), 196-199.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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