Rabbit Anti-KLF5 Recombinant Antibody (23H10L5) (CBMAB-K1115-LY)

Name: Rabbit Anti-KLF5 Recombinant Antibody (23H10L5)
SKU: CBMAB-K1115-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

23H10L5

Application

CIP, ICC, IF

Immunogen

Peptides corresponding to Human KLF5 (aa 11-24, 358-372)

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

0.09% sodium azide

Concentration

0.5 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

KLF5

Introduction

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Entrez Gene ID

688

UniProt ID

Q13887

Alternative Names

Kruppel Like Factor 5; Intestinal-Enriched Krueppel-Like Factor; Kruppel-Like Factor 5 (Intestinal); (Intestinal Kruppel-Like Factor; Colon Krueppel-Like Factor; Transcription Factor BTEB2; BTE-Binding Protein 2; BTEB2; CKLF; IKLF;

Function

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Biological Process

AngiogenesisIEA:Ensembl
Cell-cell signaling via exosomeManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular response to leukemia inhibitory factorIEA:Ensembl
Intestinal epithelial cell developmentIEA:Ensembl
Microvillus assemblyIEA:Ensembl
Myotube differentiation involved in skeletal muscle regenerationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Positive regulation of fat cell differentiationIEA:Ensembl
Positive regulation of pri-miRNA transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Positive regulation of transcription by transcription factor localizationIEA:Ensembl
Regulation of microvillus assemblyIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Satellite cell activation involved in skeletal muscle regenerationIEA:Ensembl
Skeletal muscle satellite cell differentiationIEA:Ensembl

Cellular Location

Nucleus

PTM

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Palioura, D., Lazou, A., & Drosatos, K. (2022). Krüppel-like factor (KLF) 5: An emerging foe of cardiovascular health. Journal of Molecular and Cellular Cardiology, 163, 56-66.

Jiang, D., Qiu, T., Peng, J., Li, S., Tala, Ren, W., ... & Chen, C. (2022). YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer. Cell Death & Differentiation, 29(6), 1283-1295.

Li, J. C., Chen, Q. H., Jian, R., Zhou, J. R., Xu, Y., Lu, F., ... & Zhang, H. (2021). The partial role of KLF4 and KLF5 in gastrointestinal tumors. Gastroenterology Research and Practice, 2021.

Jia, X., Chen, H., Ren, Y., Gao, N., Feng, H., Huang, W., ... & Yu, H. (2021). BAP1 antagonizes WWP1-mediated transcription factor KLF5 ubiquitination and inhibits autophagy to promote melanoma progression. Experimental Cell Research, 402(1), 112506.

Ma, J. B., Bai, J. Y., Zhang, H. B., Jia, J., Shi, Q., Yang, C., ... & Guo, P. (2020). KLF5 inhibits STAT3 activity and tumor metastasis in prostate cancer by suppressing IGF1 transcription cooperatively with HDAC1. Cell death & disease, 11(6), 466.

An, T., Dong, T., Zhou, H., Chen, Y., Zhang, J., Zhang, Y. U., ... & Yang, X. (2019). The transcription factor Krüppel-like factor 5 promotes cell growth and metastasis via activating PI3K/AKT/Snail signaling in hepatocellular carcinoma. Biochemical and biophysical research communications, 508(1), 159-168.

Azami, T., Matsumoto, K., Jeon, H., Waku, T., Muratani, M., Niwa, H., ... & Ema, M. (2018). Klf5 suppresses ERK signaling in mouse pluripotent stem cells. PLoS One, 13(11), e0207321.

Zhang, X., Choi, P. S., Francis, J. M., Gao, G. F., Campbell, J. D., Ramachandran, A., ... & Meyerson, M. (2018). Somatic superenhancer duplications and hotspot mutations lead to oncogenic activation of the KLF5 transcription factor. Cancer discovery, 8(1), 108-125.

Ma, Y., Wang, Q., Liu, F., Ma, X., Wu, L., Guo, F., ... & Qin, G. (2018). KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway. Oncology reports, 40(5), 2608-2618.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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