Mouse Anti-KLHL13 Recombinant Antibody (CBT4530) (V2LY-0625-LY2466)

Name: Mouse Anti-KLHL13 Recombinant Antibody (CBT4530)
SKU: V2LY-0625-LY2466
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-KLHL13 Recombinant Antibody (CBT4530) (V2LY-0625-LY2466) in WB

Mouse Anti-KLHL13 Recombinant Antibody (CBT4530) (V2LY-0625-LY2466) in IF

Mouse Anti-KLHL13 Recombinant Antibody (CBT4530) (V2LY-0625-LY2466) in IHC

Mouse Anti-KLHL13 Recombinant Antibody (CBT4530) (V2LY-0625-LY2466) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4530

Application

WB, IHC, ICC, FC

Immunogen

Purified recombinant fragment of human KLHL13 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kelch Like Family Member 13

Entrez Gene ID

90293

UniProt ID

Q9P2N7

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.

Biological Process

Cell cycleIEA:UniProtKB-KW
Cell divisionIEA:UniProtKB-KW
Protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cul3-RING ubiquitin ligase complex: Cytosol

Li, Q., Nan, D., Rao, C., Tang, Z., Yang, W., Wu, P., ... & Mao, X. (2023). Burkholderia pseudomallei BipD hijacks host KLHL9/KLHL13/CUL3 E3 ligase to ubiquitinate IMMT that initiates mitophagy to evade killing.

Frendo-Cumbo, S., Jaldin-Fincati, J. R., Coyaud, E., Laurent, E. M., Townsend, L. K., Tan, J. M., ... & Klip, A. (2019). Deficiency of the autophagy gene ATG16L1 induces insulin resistance through KLHL9/KLHL13/CUL3-mediated IRS1 degradation. Journal of Biological Chemistry, 294(44), 16172-16185.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-KLHL13 Recombinant Antibody (8D1) (CAT#: CBMAB-K1181-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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