Rabbit Anti-KLKB1 Recombinant Antibody (
9F6) (V2LY-0725-LY1233)

Name: Rabbit Anti-KLKB1 Recombinant Antibody (9F6)
SKU: V2LY-0725-LY1233
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-KLKB1 Recombinant Antibody (9F6) (V2LY-0725-LY1233) in IHC

IHC image of V2LY-0725-LY1233 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

9F6

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from human KLKB1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kallikrein B1

Entrez Gene ID

3818

UniProt ID

P03952

Function

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Biological Process

Blood coagulationIEA:UniProtKB-KW
Blood coagulation, intrinsic pathwayIDA:ComplexPortal
Factor XII activationManual Assertion Based On ExperimentTAS:BHF-UCL
FibrinolysisIEA:UniProtKB-KW
Plasminogen activationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of fibrinolysisManual Assertion Based On ExperimentIDA:BHF-UCL
ProteolysisManual Assertion Based On ExperimentIDA:ComplexPortal
Zymogen activationManual Assertion Based On ExperimentIDA:ComplexPortal

Cellular Location

Secreted

Involvement in disease

Prekallikrein deficiency (PKK deficiency):
This disorder is a blood coagulation defect.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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