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Recombinant Rat Anti-KSR1 Recombinant Antibody (MINT/1/14) (CBMAB-XB0728-YC)

Provided herein is a Rat Recombinant Antibody against Kinase Suppressor Of Ras 1. The antibody can be used for immunoassay techniques, such as IHC, WB.
See all KSR1 antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse, Rat
Clone
MINT/1/14
Antibody Isotype
IgG2a
Application
IHC, WB

Basic Information

Immunogen
Recombinant human KSR protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Kinase Suppressor Of Ras 1
Introduction
Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP.
Entrez Gene ID
Human8844
Mouse16706
Rat108348076
UniProt ID
HumanQ8IVT5
MouseQ61097
RatD3ZHL1
Alternative Names
Kinase Suppressor Of Ras 1; KSR; Kinase Suppressor Of Ras; RSU2;
Function
Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity).
Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126).
Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity).
Its kinase activity is unsure (By similarity).
Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
Biological Process
cAMP-mediated signalingISS:UniProtKB
Positive regulation of MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Ras protein signal transductionISS:UniProtKB
Regulation of cell population proliferationISS:UniProtKB
Regulation of MAP kinase activityISS:UniProtKB
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Membrane 1 Publication
Cell membrane
Cell projection, ruffle membrane
Endoplasmic reticulum membrane
In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
PTM
Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).

Liu, Z., Krstic, A., Neve, A., Casalou, C., Rauch, N., Wynne, K., ... & Kolch, W. (2023). Kinase Suppressor of RAS 1 (KSR1) maintains the transformed phenotype of BRAFV600E mutant human melanoma cells. International Journal of Molecular Sciences, 24(14), 11821.

Daley, B. R., Vieira, H. M., Rao, C., Hughes, J. M., Beckley, Z. M., Huisman, D. H., ... & Kortum, R. L. (2023). SOS1 and KSR1 modulate MEK inhibitor responsiveness to target resistant cell populations based on PI3K and KRAS mutation status. Proceedings of the National Academy of Sciences, 120(47), e2313137120.

Germino, E. A., Miller, J. P., Diehl, L., Swanson, C. J., Durinck, S., Modrusan, Z., ... & Shaw, A. S. (2018). Homozygous KSR1 deletion attenuates morbidity but does not prevent tumor development in a mouse model of RAS-driven pancreatic cancer. PloS one, 13(3), e0194998.

Bai, Y., Han, G., Guo, K., Yu, L., Du, X., & Xu, Y. (2018). Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury. Acta Biochimica et Biophysica Sinica, 50(8), 807-816.

Stevens, P. D., Wen, Y. A., Xiong, X., Zaytseva, Y. Y., Li, A. T., Wang, C., ... & Gao, T. (2018). Erbin suppresses KSR1-mediated RAS/RAF signaling and tumorigenesis in colorectal cancer. Cancer research, 78(17), 4839-4852.

Basu, S. K., Lee, S., Salotti, J., Basu, S., Sakchaisri, K., Xiao, Z., ... & Johnson, P. F. (2018). Oncogenic RAS-induced perinuclear signaling complexes requiring KSR1 regulate signal transmission to downstream targets. Cancer research, 78(4), 891-908.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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