Rat Anti-KSR1 Recombinant Antibody (MINT/1/4) (CBMAB-K2069-LY)

Name: Rat Anti-KSR1 Recombinant Antibody (MINT/1/4)
SKU: CBMAB-K2069-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

MINT/1/4

Application

IHC-Fr, IHC-P, WB

Immunogen

Recombinant human KSR protein

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

0.09% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Kinase Suppressor Of Ras 1

Introduction

Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself.

Entrez Gene ID

Human	8844
Mouse	16706
Rat	108348076

UniProt ID

Human	Q8IVT5
Mouse	Q61097
Rat	D3ZHL1

Alternative Names

Kinase Suppressor Of Ras 1; KSR; Kinase Suppressor Of Ras; RSU2;

Function

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity).
Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126).
Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity).
Its kinase activity is unsure (By similarity).
Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).

Biological Process

cAMP-mediated signalingISS:UniProtKB
Positive regulation of MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Ras protein signal transductionISS:UniProtKB
Regulation of cell population proliferationISS:UniProtKB
Regulation of MAP kinase activityISS:UniProtKB
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Membrane 1 Publication
Cell membrane
Cell projection, ruffle membrane
Endoplasmic reticulum membrane
In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.

PTM

Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).

Liu, Z., Krstic, A., Neve, A., Casalou, C., Rauch, N., Wynne, K., ... & Kolch, W. (2023). Kinase Suppressor of RAS 1 (KSR1) maintains the transformed phenotype of BRAFV600E mutant human melanoma cells. International Journal of Molecular Sciences, 24(14), 11821.

Daley, B. R., Vieira, H. M., Rao, C., Hughes, J. M., Beckley, Z. M., Huisman, D. H., ... & Kortum, R. L. (2023). SOS1 and KSR1 modulate MEK inhibitor responsiveness to target resistant cell populations based on PI3K and KRAS mutation status. Proceedings of the National Academy of Sciences, 120(47), e2313137120.

Germino, E. A., Miller, J. P., Diehl, L., Swanson, C. J., Durinck, S., Modrusan, Z., ... & Shaw, A. S. (2018). Homozygous KSR1 deletion attenuates morbidity but does not prevent tumor development in a mouse model of RAS-driven pancreatic cancer. PloS one, 13(3), e0194998.

Bai, Y., Han, G., Guo, K., Yu, L., Du, X., & Xu, Y. (2018). Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury. Acta Biochimica et Biophysica Sinica, 50(8), 807-816.

Stevens, P. D., Wen, Y. A., Xiong, X., Zaytseva, Y. Y., Li, A. T., Wang, C., ... & Gao, T. (2018). Erbin suppresses KSR1-mediated RAS/RAF signaling and tumorigenesis in colorectal cancer. Cancer research, 78(17), 4839-4852.

Basu, S. K., Lee, S., Salotti, J., Basu, S., Sakchaisri, K., Xiao, Z., ... & Johnson, P. F. (2018). Oncogenic RAS-induced perinuclear signaling complexes requiring KSR1 regulate signal transmission to downstream targets. Cancer research, 78(4), 891-908.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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