Human Recombinant KSR1 protein, GST Tag (V2LY-0526-LY5219)

Name: Human Recombinant KSR1 protein, GST Tag
SKU: V2LY-0526-LY5219
Rating: 5 (1 reviews)

See all KSR1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant KSR1 protein, GST Tag consist of Amino Acid: 335-end and predicts a molecular mass of 105 kDa.

Molecule Mass

105 kDa

Sequence

Amino Acid: 335-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Kinase Suppressor Of Ras 1

Function

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity).
Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126).
Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity).
Its kinase activity is unsure (By similarity).
Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).

Biological Process

cAMP-mediated signalingISS:UniProtKB
Positive regulation of MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Ras protein signal transductionISS:UniProtKB
Regulation of cell population proliferationISS:UniProtKB
Regulation of MAP kinase activityISS:UniProtKB
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Membrane 1 Publication
Cell membrane
Cell projection, ruffle membrane
Endoplasmic reticulum membrane
In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.

PTM

Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).

Liu, Z., Krstic, A., Neve, A., Casalou, C., Rauch, N., Wynne, K., ... & Kolch, W. (2023). Kinase Suppressor of RAS 1 (KSR1) maintains the transformed phenotype of BRAFV600E mutant human melanoma cells. International Journal of Molecular Sciences, 24(14), 11821.

Daley, B. R., Vieira, H. M., Rao, C., Hughes, J. M., Beckley, Z. M., Huisman, D. H., ... & Kortum, R. L. (2023). SOS1 and KSR1 modulate MEK inhibitor responsiveness to target resistant cell populations based on PI3K and KRAS mutation status. Proceedings of the National Academy of Sciences, 120(47), e2313137120.

Germino, E. A., Miller, J. P., Diehl, L., Swanson, C. J., Durinck, S., Modrusan, Z., ... & Shaw, A. S. (2018). Homozygous KSR1 deletion attenuates morbidity but does not prevent tumor development in a mouse model of RAS-driven pancreatic cancer. PloS one, 13(3), e0194998.

Bai, Y., Han, G., Guo, K., Yu, L., Du, X., & Xu, Y. (2018). Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury. Acta Biochimica et Biophysica Sinica, 50(8), 807-816.

Stevens, P. D., Wen, Y. A., Xiong, X., Zaytseva, Y. Y., Li, A. T., Wang, C., ... & Gao, T. (2018). Erbin suppresses KSR1-mediated RAS/RAF signaling and tumorigenesis in colorectal cancer. Cancer research, 78(17), 4839-4852.

Basu, S. K., Lee, S., Salotti, J., Basu, S., Sakchaisri, K., Xiao, Z., ... & Johnson, P. F. (2018). Oncogenic RAS-induced perinuclear signaling complexes requiring KSR1 regulate signal transmission to downstream targets. Cancer research, 78(4), 891-908.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant KSR1 protein, GST Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rat Anti-KSR1 Recombinant Antibody (MINT/1/4) (CAT#: CBMAB-K2069-LY)

Rat Anti-KSR1 Recombinant Antibody (3H2582) (CAT#: CBMAB-K2066-LY)

Rabbit Anti-KSR1 Recombinant Antibody (10F647) (CAT#: CBMAB-K2064-LY)

Rabbit Anti-KSR1 Recombinant Antibody (CBLY1-165) (CAT#: CBMAB-K2063-LY)

Rabbit Anti-KSR1 Recombinant Antibody (EP2358Y) (CAT#: CBMAB-K2068-LY)

Rabbit Anti-KSR1 Recombinant Antibody (CBLY1-164) (CAT#: CBMAB-K2062-LY)

Recombinant Rat Anti-KSR1 Recombinant Antibody (MINT/1/14) (CAT#: CBMAB-XB0728-YC)

Mouse Anti-KSR1 Recombinant Antibody (6C7) (CAT#: CBMAB-K2067-LY)

Mouse Anti-KSR1 Recombinant Antibody (15/KSR-1) (CAT#: CBMAB-K2065-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry