Mouse Anti-KSR2 Recombinant Antibody (3F11) (CBMAB-K2072-LY)

Name: Mouse Anti-KSR2 Recombinant Antibody (3F11)
SKU: CBMAB-K2072-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3F11

Application

ELISA, WB

Immunogen

KSR2 (NP_775869, 411 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: PAPPLPPSAT PPSPLHPSPQ CTRQQKNFNL PASHYYKYKQ QFIFPDVVPV PETPTRAPQV ILHPVTSNPI LEGNPLLQIE VEPTSENEEV

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

kinase suppressor of ras 2

Introduction

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

Entrez Gene ID

283455

UniProt ID

Q6VAB6

Alternative Names

Kinase Suppressor Of Ras 2; EC 2.7.11.1; HKSR2;

Function

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed:29433126).
Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

Biological Process

Calcium-mediated signalingManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Ras protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Membrane

PTM

Phosphorylated on Ser-474 by MARK3.

Huisman, D. H., Frodyma, D. E., Svoboda, R. A., Vieira, H. M., Chatterjee, D., Skupa, S., ... & Lewis, R. E. (2022). Kinase Suppressor of Ras 2 regulates small-cell lung carcinoma tumor propagating cells. bioRxiv, 2022-02.

Gao, C., Wang, S. W., Lu, J. C., Chai, X. Q., Li, Y. C., Zhang, P. F., ... & Fan, J. (2022). KSR2-14–3-3ζ complex serves as a biomarker and potential therapeutic target in sorafenib-resistant hepatocellular carcinoma. Biomarker Research, 10(1), 25.

Gomez, G. A., Rundle, C. H., Xing, W., Kesavan, C., Pourteymoor, S., Lewis, R. E., ... & Mohan, S. (2022). Contrasting effects of Ksr2, an obesity gene, on trabecular bone volume and bone marrow adiposity. Elife, 11, e82810.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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