Mouse Anti-LGR5 Recombinant Antibody (3E11) (CBMAB-L1347-YJ)

Name: Mouse Anti-LGR5 Recombinant Antibody (3E11)
SKU: CBMAB-L1347-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3E11

Application

Immunogen

Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in 293T Cell.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 250-550

More Infomation

Target

Full Name

LGR5

Introduction

LGR5 is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. LGR5 is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein functions in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

8549

UniProt ID

O75473

Alternative Names

FEX; HG38; GPR49; GPR67; GRP49

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Biological Process

Activation of adenylate cyclase activityManual Assertion Based On ExperimentIBA:GO_Central
Adenylate cyclase-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Epithelial cell proliferation involved in renal tubule morphogenesisIEA:Ensembl
G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Hair follicle developmentIEA:Ensembl
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Inner ear developmentIEA:Ensembl
Oocyte differentiationIEA:Ensembl
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell population proliferationIEA:Ensembl

Cellular Location

Cell membrane
Golgi apparatus, trans-Golgi network membrane
Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).

Topology

Extracellular: 22-561
Helical: 562-582
Cytoplasmic: 583-593
Helical: 594-614
Extracellular: 615-638
Helical: 639-659
Cytoplasmic: 660-682
Helical: 683-703
Extracellular: 704-722
Helical: 723-743
Cytoplasmic: 744-767
Helical: 768-788
Extracellular: 789-802
Helical: 803-823
Cytoplasmic: 824-907

Hahn, S., Kim, M. S., Choi, S. Y., Jeong, S., Jee, J., Kim, H. K., ... & Yoo, J. (2019). Leucine-rich repeat-containing G-protein coupled receptor 5 enriched organoids under chemically-defined growth conditions. Biochemical and biophysical research communications, 508(2), 430-439.

Nakajima, T., Uehara, T., Kobayashi, Y., Kinugawa, Y., Yamanoi, K., Maruyama, Y., ... & Ota, H. (2018). Leucine‐rich repeat‐containing G‐protein‐coupled receptor 5 expression and clinicopathological features of colorectal neuroendocrine neoplasms. Pathology international, 68(8), 467-472.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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