Human Recombinant LGR5 protein, His Tag (V2LY-0526-LY5291)

Name: Human Recombinant LGR5 protein, His Tag
SKU: V2LY-0526-LY5291
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant LGR5 protein, His Tag consist of Amino Acid: 32-544 and predicts a molecular mass of 53.71 kDa.

Molecule Mass

53.71 kDa

Sequence

Amino Acid: 32-544

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

LGR5

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Biological Process

Activation of adenylate cyclase activityManual Assertion Based On ExperimentIBA:GO_Central
Adenylate cyclase-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Epithelial cell proliferation involved in renal tubule morphogenesisIEA:Ensembl
G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Hair follicle developmentIEA:Ensembl
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Inner ear developmentIEA:Ensembl
Oocyte differentiationIEA:Ensembl
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell population proliferationIEA:Ensembl

Cellular Location

Cell membrane
Golgi apparatus, trans-Golgi network membrane
Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).

Topology

Extracellular: 22-561
Helical: 562-582
Cytoplasmic: 583-593
Helical: 594-614
Extracellular: 615-638
Helical: 639-659
Cytoplasmic: 660-682
Helical: 683-703
Extracellular: 704-722
Helical: 723-743
Cytoplasmic: 744-767
Helical: 768-788
Extracellular: 789-802
Helical: 803-823
Cytoplasmic: 824-907

Hahn, S., Kim, M. S., Choi, S. Y., Jeong, S., Jee, J., Kim, H. K., ... & Yoo, J. (2019). Leucine-rich repeat-containing G-protein coupled receptor 5 enriched organoids under chemically-defined growth conditions. Biochemical and biophysical research communications, 508(2), 430-439.

Nakajima, T., Uehara, T., Kobayashi, Y., Kinugawa, Y., Yamanoi, K., Maruyama, Y., ... & Ota, H. (2018). Leucine‐rich repeat‐containing G‐protein‐coupled receptor 5 expression and clinicopathological features of colorectal neuroendocrine neoplasms. Pathology international, 68(8), 467-472.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LGR5-VLP protein, Flag & His-Strep Tag (CAT#: V2LY-0526-LY5292)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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