Mouse Anti-LHX5 Recombinant Antibody (2B4) (CBMAB-A4977-LY)

Name: Mouse Anti-LHX5 Recombinant Antibody (2B4)
SKU: CBMAB-A4977-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2B4

Application

ELISA

Immunogen

LHX5 (NP_071758, 136 a.a. ~ 235 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

LIM homeobox 5

Introduction

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in the control of differentiation and development of the forebrain. In mice, this protein is essential for the regulation of precursor cell proliferation and the control of neuronal differentiation and migration during hippocampal development. This protein is involved in learning and motor functions in adult mice. [provided by RefSeq]

Entrez Gene ID

64211

UniProt ID

Q9H2C1

Alternative Names

MGC129689

Function

Plays an essential role in the regulation of neuronal differentiation and migration during development of the central nervous system.

Biological Process

Cell proliferation in forebrainIEA:Ensembl
Cerebellar Purkinje cell differentiationIEA:Ensembl
Cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferationIEA:Ensembl
Forebrain neuron differentiationIEA:Ensembl
Hippocampus developmentIEA:Ensembl
Neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of transcription, DNA-templatedIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Spinal cord association neuron differentiationIEA:Ensembl

Cellular Location

Nucleus

Molina, M. D., Abduljabbar, D., Guixeras, A., Fraguas, S., & Cebrià, F. (2023). LIM-HD transcription factors control axial patterning and specify distinct neuronal and intestinal cell identities in planarians. Open Biology, 13(12), 230327.

Singh, N., Singh, D., & Modi, D. (2022). LIM homeodomain (LIM-HD) genes and their co-regulators in developing reproductive system and disorders of sex development. Sexual Development, 16(2-3), 147-161.

Gao, P., Sun, N., Zhao, T., Sun, Y., Gu, J., Ma, D., ... & Qi, X. (2022). Identification of prognostic indicators, diagnostic markers, and possible therapeutic targets among LIM homeobox transcription factors in breast cancer. Cancer Innovation, 1(3), 252-269.

Akhir, M. K. A. M., Choy, C. S., Abdullah, M. A., Abd Ghani, F., Veerakumarasivam, A., & Hussin, H. (2020). The role of ISL1 and LHX5 LIM homeobox genes in bladder tumourigenesis. The Malaysian journal of medical sciences: MJMS, 27(1), 37.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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