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Rabbit Anti-LIN28B Recombinant Antibody (CBYJL-1746) (CBMAB-L1651-YJ)

Provided herein is a Rabbit monoclonal antibody, which binds to Lin-28 Homolog B (LIN28B). The antibody can be used for immunoassay techniques, such as WB, IP.
See all LIN28B antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBYJL-1746
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LIN28B
Introduction
LIN28B belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. LIN28B is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. Diseases associated with LIN28B include Idiopathic Central Precocious Puberty and Oral Cavity Cancer. Among its related pathways are Translational Control and Validated targets of C-MYC transcriptional activation.
Entrez Gene ID
389421
UniProt ID
Q6ZN17
Alternative Names
CSDD2
Function
Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463).
Does not act on pri-miR21 (PubMed:22118463).
The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396).
This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463).
Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity).
When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Biological Process
miRNA catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of pre-miRNA processingManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of primary miRNA processingManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of miRNA catabolic processManual Assertion Based On ExperimentIMP:CACAO
Pre-miRNA processingManual Assertion Based On ExperimentIMP:UniProtKB
RNA 3'-end processingManual Assertion Based On ExperimentIMP:UniProtKB
RNA destabilizationManual Assertion Based On ExperimentIDA:CACAO
Cellular Location
Nucleus
Nucleus, nucleolus
Cytoplasm
Predominantly nucleolar (PubMed:22118463).
In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064).

Qi, M., Xia, Y., Wu, Y., Zhang, Z., Wang, X., Lu, L., ... & Zhan, L. (2022). Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression. Nature communications, 13(1), 897.

Missios, P., da Rocha, E. L., Pearson, D. S., Philipp, J., Aleman, M. M., Pirouz, M., ... & Daley, G. Q. (2021). LIN28B alters ribosomal dynamics to promote metastasis in MYCN-driven malignancy. The Journal of Clinical Investigation, 131(22).

Yang, Y., Wei, Q., Tang, Y., Wang, Y., Luo, Q., Zhao, H., ... & Yi, P. (2020). Loss of hnRNPA2B1 inhibits malignant capability and promotes apoptosis via down-regulating Lin28B expression in ovarian cancer. Cancer letters, 475, 43-52.

Keskin, T., Bakaric, A., Waszyk, P., Boulay, G., Torsello, M., Cornaz-Buros, S., ... & Stamenkovic, I. (2020). LIN28B underlies the pathogenesis of a subclass of Ewing sarcoma. Cell reports, 30(13), 4567-4583.

Lovnicki, J., Gan, Y., Feng, T., Li, Y., Xie, N., Ho, C. H., ... & Dong, X. (2020). LIN28B promotes the development of neuroendocrine prostate cancer. The Journal of clinical investigation, 130(10), 5338-5348.

Basak, A., Munschauer, M., Lareau, C. A., Montbleau, K. E., Ulirsch, J. C., Hartigan, C. R., ... & Sankaran, V. G. (2020). Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A translation. Nature genetics, 52(2), 138-145.

Zhang, J., Xu, A., Miao, C., Yang, J., Gu, M., & Song, N. (2019). Prognostic value of Lin28A and Lin28B in various human malignancies: a systematic review and meta-analysis. Cancer cell international, 19(1), 1-8.

Wang, S., Chim, B., Su, Y., Khil, P., Wong, M., Wang, X., ... & Muljo, S. A. (2019). Enhancement of LIN28B-induced hematopoietic reprogramming by IGF2BP3. Genes & development, 33(15-16), 1048-1068.

Guo, W., Hu, Z., Bao, Y., Li, Y., Li, S., Zheng, Q., ... & Huang, S. (2018). A LIN28B tumor-specific transcript in cancer. Cell reports, 22(8), 2016-2025.

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For research use only. Not intended for any clinical use.

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