Mouse Lin28b ELISA Kit (V2LY-0626-LY615)

Name: Mouse Lin28b ELISA Kit
SKU: V2LY-0626-LY615
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.14 ng/mL

Detection Range

0.25-16 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

26.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

LIN28B

Function

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463).
Does not act on pri-miR21 (PubMed:22118463).
The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396).
This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463).
Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity).
When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Biological Process

miRNA catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of pre-miRNA processingManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of primary miRNA processingManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of miRNA catabolic processManual Assertion Based On ExperimentIMP:CACAO
Pre-miRNA processingManual Assertion Based On ExperimentIMP:UniProtKB
RNA 3'-end processingManual Assertion Based On ExperimentIMP:UniProtKB
RNA destabilizationManual Assertion Based On ExperimentIDA:CACAO

Cellular Location

Nucleus
Nucleus, nucleolus
Cytoplasm
Predominantly nucleolar (PubMed:22118463).
In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064).

Qi, M., Xia, Y., Wu, Y., Zhang, Z., Wang, X., Lu, L., ... & Zhan, L. (2022). Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression. Nature communications, 13(1), 897.

Missios, P., da Rocha, E. L., Pearson, D. S., Philipp, J., Aleman, M. M., Pirouz, M., ... & Daley, G. Q. (2021). LIN28B alters ribosomal dynamics to promote metastasis in MYCN-driven malignancy. The Journal of Clinical Investigation, 131(22).

Yang, Y., Wei, Q., Tang, Y., Wang, Y., Luo, Q., Zhao, H., ... & Yi, P. (2020). Loss of hnRNPA2B1 inhibits malignant capability and promotes apoptosis via down-regulating Lin28B expression in ovarian cancer. Cancer letters, 475, 43-52.

Keskin, T., Bakaric, A., Waszyk, P., Boulay, G., Torsello, M., Cornaz-Buros, S., ... & Stamenkovic, I. (2020). LIN28B underlies the pathogenesis of a subclass of Ewing sarcoma. Cell reports, 30(13), 4567-4583.

Lovnicki, J., Gan, Y., Feng, T., Li, Y., Xie, N., Ho, C. H., ... & Dong, X. (2020). LIN28B promotes the development of neuroendocrine prostate cancer. The Journal of clinical investigation, 130(10), 5338-5348.

Basak, A., Munschauer, M., Lareau, C. A., Montbleau, K. E., Ulirsch, J. C., Hartigan, C. R., ... & Sankaran, V. G. (2020). Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A translation. Nature genetics, 52(2), 138-145.

Zhang, J., Xu, A., Miao, C., Yang, J., Gu, M., & Song, N. (2019). Prognostic value of Lin28A and Lin28B in various human malignancies: a systematic review and meta-analysis. Cancer cell international, 19(1), 1-8.

Wang, S., Chim, B., Su, Y., Khil, P., Wong, M., Wang, X., ... & Muljo, S. A. (2019). Enhancement of LIN28B-induced hematopoietic reprogramming by IGF2BP3. Genes & development, 33(15-16), 1048-1068.

Guo, W., Hu, Z., Bao, Y., Li, Y., Li, S., Zheng, Q., ... & Huang, S. (2018). A LIN28B tumor-specific transcript in cancer. Cell reports, 22(8), 2016-2025.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Chicken LIN28B ELISA Kit (CAT#: V2LY-0626-LY190)

Human LIN28B ELISA Kit (CAT#: V2LY-0626-LY882)

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Mouse Anti-LIN28B Recombinant Antibody (CBYCL-351) (CAT#: CBMAB-L0252-YC)

Mouse Anti-LIN28B Recombinant Antibody (A910) (CAT#: CBMAB-AP11491LY)

Human LIN28B ELISA Kit (CAT#: V2LY-0626-LY882)

Chicken LIN28B ELISA Kit (CAT#: V2LY-0626-LY190)

Isotype control

For research use only. Not intended for any clinical use.

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