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Mouse Anti-LLGL1 Recombinant Antibody (5G2) (CBMAB-L1733-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Llgl1 Scribble Cell Polarity Complex Component (LLGL1). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all LLGL1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5G2
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
LLGL1 (NP_004131, 911 a.a. ~ 1010 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: GIASCVFTRH GQGFYLISPS EFERFSLSAR NITEPLCSLD INWPRDATQA SYRIRESPKL SQANGTPSIL LAPQSLDGSP DPAHSMGPDT PEPPEAALSP.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 911-1010

Target

Full Name
lethal giant larvae homolog 1 (Drosophila)
Introduction
LLGL1 is similar to a tumor suppressor in Drosophila. LLGL1 is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with LLGL1 include Smith-Magenis Syndrome and Adiaspiromycosis. Among its related pathways are Actin Nucleation by ARP-WASP Complex and PI3K-Akt signaling pathway.
Entrez Gene ID
3996
UniProt ID
Q15334
Alternative Names
DLG4; HUGL; LLGL; Lgl1; Mgl1; HUGL1; HUGL-1
Function
Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.
Biological Process
AxonogenesisISS:UniProtKB
Cortical actin cytoskeleton organizationManual Assertion Based On ExperimentIDA:UniProtKB
Establishment of spindle orientationManual Assertion Based On ExperimentIBA:GO_Central
Establishment or maintenance of epithelial cell apical/basal polarity1 PublicationIC:ComplexPortal
ExocytosisIEA:UniProtKB-KW
Golgi to plasma membrane transportISS:UniProtKB
Protein-containing complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of establishment or maintenance of cell polarityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of Notch signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Regulation of protein secretionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Early endosome membrane
Golgi apparatus, trans-Golgi network membrane
Golgi apparatus membrane
Cell projection, axon
Cytoplasm, cytoskeleton
Localized to the lateral membrane during the polarization and formation cell-cell contacts. Enriched in developing axons (By similarity).
PTM
Phosphorylated at least at Ser-663 by PRKCI.

Eifert, T., Hsu, C. J., Becker, A. L., Graessle, S., Horne, A., Bemmann, F., ... & Heidel, F. H. (2023). Cell fate determinant Llgl1 is required for propagation of acute myeloid leukemia. Leukemia, 37(10), 2027-2035.

Bii, V. M., Rudoy, D., Klezovitch, O., & Vasioukhin, V. (2023). Lethal giant larvae gene family (Llgl1 and Llgl2) functions as a tumor suppressor in mouse skin epidermis. bioRxiv, 2023-03.

Pollitt, E. J., Derrick, C. J., Sánchez-Posada, J., & Noël, E. S. (2023). Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the trabeculating cardiac ventricle. bioRxiv, 2023-08.

Eifert, T., Schnoeder, T. M., Hsu, C. J., Becker, A. L., Zhang, Q., Mohr, J., ... & Heidel, F. H. (2022). Loss of Cell Fate Determinant LLGL1 Impairs Leukemia Development in Acute Myeloid Leukemia. Blood, 140(Supplement 1), 8680-8681.

Liu, N., Cui, W., Chen, M., Zhang, X., Song, X., & Pan, C. (2021). A 21-bp indel within the LLGL1 gene is significantly associated with litter size in goat. Animal Biotechnology, 32(2), 213-218.

Flinn, M. A., Otten, C., Brandt, Z. J., Bostrom, J. R., Kenarsary, A., Wan, T. C., ... & Link, B. A. (2020). Llgl1 regulates zebrafish cardiac development by mediating Yap stability in cardiomyocytes. Development, 147(16), dev193581.

Desuki, A., Staib, F., Gockel, I., Moehler, M., Lang, H., Biesterfeld, S., ... & Schimanski, C. C. (2019). Loss of LLGL1 expression correlates with diffuse gastric cancer and distant peritoneal metastases. Canadian Journal of Gastroenterology and Hepatology, 2019.

Dash, B. P., Schnöder, T. M., Kathner, C., Mohr, J., Weinert, S., Herzog, C., ... & Heidel, F. H. (2018). Diverging impact of cell fate determinants Scrib and Llgl1 on adhesion and migration of hematopoietic stem cells. Journal of Cancer Research and Clinical Oncology, 144, 1933-1944.

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For research use only. Not intended for any clinical use.

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