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Mouse Anti-LRAT Recombinant Antibody (4E12) (CBMAB-L2006-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Lecithin Retinol Acyltransferase (LRAT). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all LRAT antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4E12
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
LRAT (NP_004735.2, 33 a.a. ~ 132 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: DKGRNSFYET SSFHRGDVLE VPRTHLTHYG IYLGDNRVAH MMPDILLALT DDMGRTQKVV SNKRLILGVI VKVASIRVDT VEDFAYGANI LVNHLDESLQ.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 33-132

Target

Full Name
lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Introduction
LRAT localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been related to early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
9227
UniProt ID
O95237
Alternative Names
LCA14; lecithin retinol acyltransferase; lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase); EC 2.3.1.135
Function
Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters (PubMed:9920938).
Retinyl esters are storage forms of vitamin A (Probable). LRAT plays a critical role in vision (Probable). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (Probable). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology (By similarity).
Biological Process
Cellular response to leukemia inhibitory factorIEA:Ensembl
Positive regulation of lipid transportIEA:Ensembl
Response to bacteriumIEA:Ensembl
Response to retinoic acidIEA:Ensembl
Response to vitamin AIEA:Ensembl
Retinoid metabolic processTAS:Reactome
Retinol metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Visual perceptionIEA:UniProtKB-KW
Vitamin A metabolic processISS:UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Rough endoplasmic reticulum
Endosome, multivesicular body
Cytoplasm, perinuclear region
Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells (By similarity).
Involvement in disease
Leber congenital amaurosis 14 (LCA14):
A severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.
Topology
Cytoplasmic: 1-194
Helical: 195-215
Lumenal: 216-230
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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