Mouse Anti-LRAT Recombinant Antibody (M34P1F10) (CBMAB-L2007-YJ)

Name: Mouse Anti-LRAT Recombinant Antibody (M34P1F10)
SKU: CBMAB-L2007-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

M34P1F10

Application

ELISA, IHC, IF, WB

Immunogen

Peptide Sequence-RDQRSVLASA (amino acids 190 -199).

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 190 -199

More Infomation

Target

Full Name

lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)

Introduction

LRAT localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been related to early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

9227

UniProt ID

O95237

Alternative Names

LCA14; lecithin retinol acyltransferase; lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase); EC 2.3.1.135

Function

Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters (PubMed:9920938).
Retinyl esters are storage forms of vitamin A (Probable). LRAT plays a critical role in vision (Probable). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (Probable). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology (By similarity).

Biological Process

Cellular response to leukemia inhibitory factorIEA:Ensembl
Positive regulation of lipid transportIEA:Ensembl
Response to bacteriumIEA:Ensembl
Response to retinoic acidIEA:Ensembl
Response to vitamin AIEA:Ensembl
Retinoid metabolic processTAS:Reactome
Retinol metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Visual perceptionIEA:UniProtKB-KW
Vitamin A metabolic processISS:UniProtKB

Cellular Location

Endoplasmic reticulum membrane
Rough endoplasmic reticulum
Endosome, multivesicular body
Cytoplasm, perinuclear region
Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells (By similarity).

Involvement in disease

Leber congenital amaurosis 14 (LCA14):
A severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.

Topology

Cytoplasmic: 1-194
Helical: 195-215
Lumenal: 216-230

Lessieur, E. M., Du, Y., Saadane, A., Liu, H., Kiser, J., & Kern, T. S. (2022). Partial deletion of Lrat inhibits prodromal characteristics of diabetic retinopathy. Investigative Ophthalmology & Visual Science, 63(7), 3590-A0045.

Ramkumar, S., Moon, J., Golczak, M., & von Lintig, J. (2021). LRAT coordinates the negative-feedback regulation of intestinal retinoid biosynthesis from β-carotene. Journal of Lipid Research, 62.

Trasino, S. E., Tang, X. H., Trujillo, C., Andres, J., Ehrt, S., & Gudas, L. J. (2020). Mycobacterium tuberculosis infection is exacerbated in mice lacking lecithin: retinol acyltransferase. Biochimica et biophysica acta. Molecular basis of disease, 1866(11), 165909.

Gauthier, M. E., Roy, S., Cantin, L., & Salesse, C. (2019). Comparison between the enzymatic activity, structure and substrate binding of mouse and human lecithin retinol acyltransferase. Biochemical and Biophysical Research Communications, 519(4), 832-837.

Molenaar, M. R., Wassenaar, T. A., Yadav, K. K., Toulmay, A., Mari, M. C., Caillon, L., ... & Helms, J. B. (2019). Lecithin: Retinol Acyl Transferase (LRAT) induces the formation of lipid droplets. bioRxiv, 733931.

Liang, X., He, Y., Chen, M., Ping, Q., & Chen, J. (2019). The association of lecithin retinol acyltransferase and the 25 (OH) D receptor with pediatric overweight and obesity. European journal of clinical nutrition, 73(12), 1626-1629.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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