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Mouse Anti-LYPLA1 Recombinant Antibody (3D5) (CBMAB-L2507-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D5
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
LYPLA1 (AAH08652.1, 66 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: NVAMPSWFDI IGLSPDSQED ESGIKQAAEN IKALIDQEVK NGIPSNRIIL GGFSQGGALS LYTALTTQQK LAGVTALSCW LPLRAS.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 66-151

Target

Full Name
lysophospholipase I
Introduction
LYPLA1 is a member of the alpha/beta hydrolase superfamily. LYPLA1 functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7.
Entrez Gene ID
10434
UniProt ID
O75608
Alternative Names
APT1; LPL1; APT-1; LPL-I; hAPT1; acyl-protein thioesterase 1; lysoPLA I; lysophospholipid-specific lysophospholipase; EC 3.1.1.5
Function
Acts as a acyl-protein thioesterase (PubMed:19439193, PubMed:20418879).
Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (PubMed:20418879).
Has depalmitoylating activity toward KCNMA1 (PubMed:22399288).
Could also depalmitoylate ADRB2 (PubMed:27481942).
Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (PubMed:19439193).
Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (By similarity).
Has much higher thioesterase activity than lysophospholipase activity (PubMed:19439193).
Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (PubMed:21393252).By Similarity5 Publications
Biological Process
Fatty acid metabolic processIEA:UniProtKB-KW
Negative regulation of Golgi to plasma membrane protein transportIEA:Ensembl
Protein depalmitoylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Cell membrane
Nucleus membrane
Endoplasmic reticulum
Shows predominantly a cytoplasmic localization with a weak expression in the cell membrane, nuclear membrane and endoplasmic reticulum.
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For research use only. Not intended for any clinical use.

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