Mouse Anti-LYPLA1 Recombinant Antibody (CBYCL-596) (CBMAB-L0497-YC)

Provided herein is a Mouse monoclonal antibody against Human LYPLA1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all LYPLA1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYCL-596

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

lysophospholipase I

Introduction

LYPLA1 is a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. pseudogenes of this gene have been defined on chromosomes 4, 6, and 7.

Entrez Gene ID

10434

UniProt ID

O75608

Alternative Names

APT-1; APT1; LPL-I; LPL1; hAPT1

Function

Acts as a acyl-protein thioesterase (PubMed:19439193, PubMed:20418879).
Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (PubMed:20418879).
Has depalmitoylating activity toward KCNMA1 (PubMed:22399288).
Could also depalmitoylate ADRB2 (PubMed:27481942).
Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (PubMed:19439193).
Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (By similarity).
Has much higher thioesterase activity than lysophospholipase activity (PubMed:19439193).
Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (PubMed:21393252).By Similarity5 Publications

Biological Process

Fatty acid metabolic processIEA:UniProtKB-KW
Negative regulation of Golgi to plasma membrane protein transportIEA:Ensembl
Protein depalmitoylationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Cell membrane
Nucleus membrane
Endoplasmic reticulum
Shows predominantly a cytoplasmic localization with a weak expression in the cell membrane, nuclear membrane and endoplasmic reticulum.

References

Zhang, X., Liu, X., Qin, L., Tang, C., Huo, Y., Wei, J., ... & Wang, B. (2023). Averrhoa carambola extractive inhibits breast cancer via regulating CEPT1 and LYPLA1. Chinese Chemical Letters, 34(3), 107618.

Zhang, Y., Zheng, M., Zhang, L., Yuan, P., Zhou, J., Wang, Y., & Wang, H. (2022). LncRNA LOXL1-AS1 facilitates the oncogenic character in cervical cancer by the miR-526b-5p/LYPLA1 Axis. Biochemical Genetics, 60(4), 1298-1312.

Qu, M., Zhao, Y., Qing, X., Zhang, X., & Li, H. (2021). Androgen‐dependent miR‐125a‐5p targets LYPLA1 and regulates global protein palmitoylation level in late‐onset hypogonadism males. Journal of Cellular Physiology, 236(6), 4738-4749.

Mohammed, A., Zhang, C., Zhang, S., Shen, Q., Li, J., Tang, Z., & Liu, H. (2019). Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1. Oncology reports, 41(2), 973-980.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-LYPLA1 Recombinant Antibody (3D5)

Rabbit Anti-LYPLA1 Recombinant Antibody (CBYJL-2248)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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