Mouse Anti-MADCAM1 Recombinant Antibody (CBFYM-1263) (CBMAB-M1422-FY)

Name: Mouse Anti-MADCAM1 Recombinant Antibody (CBFYM-1263)
SKU: CBMAB-M1422-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1263

Application

WB, IF

Immunogen

Full length human recombinant protein of human MADCAM1(NP_570116) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA

Preservative

0.02% Sodium azide

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mucosal Vascular Addressin Cell Adhesion Molecule 1

Introduction

The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1, L-selectin, and VLA-4 on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.

Entrez Gene ID

8174

UniProt ID

Q13477

Alternative Names

Mucosal Vascular Addressin Cell Adhesion Molecule 1; HMAdCAM-1; MAdCAM-1; Mucosal Addressin Cell Adhesion Molecule-1; Mucosal Addressin Cell Adhesion Molecule 1; MACAM1

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Biological Process

Cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Cell-matrix adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of leukocyte migrationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of lymphocyte migrationIEA:InterPro
Receptor clusteringManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Membrane

Topology

Extracellular: 19-317
Helical: 318-338
Cytoplasmic: 339-382

PTM

The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2023). MAdCAM‐1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals. International Journal of Cancer.

Chelvanambi, M., & Wargo, J. A. (2023). MAdCAM-1: a newly identified microbial'gut check'for T cells. Trends in Immunology.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2022). Targeting MAdCAM-1 can prevent colitic cancer progression by suppressing immune cell infiltration and inflammatory signals. medRxiv, 2022-12.

Denton, A. E., Dooley, J., Cinti, I., Silva-Cayetano, A., Fra-Bido, S., Innocentin, S., ... & Linterman, M. A. (2022). Targeting TLR4 during vaccination boosts MAdCAM-1+ lymphoid stromal cell activation and promotes the aged germinal center response. Science immunology, 7(71), eabk0018.

Reinisch, W., Sandborn, W. J., Danese, S., Hébuterne, X., Kłopocka, M., Tarabar, D., ... & Vermeire, S. (2021). Long-term safety and efficacy of the Anti-MAdCAM-1 monoclonal antibody ontamalimab [SHP647] for the treatment of ulcerative colitis: the open-label study TURANDOT II. Journal of Crohn's and Colitis, 15(6), 938-949.

Schippers, A., Hübel, J., Heymann, F., Clahsen, T., Eswaran, S., Schlepütz, S., ... & Wagner, N. (2021). MAdCAM-1/α4β7 integrin-mediated lymphocyte/endothelium interactions exacerbate acute immune-mediated hepatitis in mice. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1227-1250.

Truffi, M., Sevieri, M., Morelli, L., Monieri, M., Mazzucchelli, S., Sorrentino, L., ... & Corsi, F. (2020). Anti-MAdCAM-1-conjugated nanocarriers delivering quantum dots enable specific imaging of inflammatory bowel disease. International Journal of Nanomedicine, 8537-8552.

Natoni, A., Farrell, M. L., Harris, S., Falank, C., Kirkham-McCarthy, L., Macauley, M. S., ... & O’Dwyer, M. (2020). Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model. Haematologica, 105(2), 457.

Tian, Y., Guo, Y., Zhu, P., Zhang, D., Liu, S., Tang, M., ... & Zhu, X. (2019). TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1. Aging (Albany NY), 11(19), 8623.

Kuhbandner, K., Hammer, A., Haase, S., Terbrack, E., Hoffmann, A., Schippers, A., ... & Stüve, O. (2019). MAdCAM-1-mediated intestinal lymphocyte homing is critical for the development of active experimental autoimmune encephalomyelitis. Frontiers in immunology, 10, 903.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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