Rabbit Anti-MADCAM1 Recombinant Antibody (
12B5) (V2LY-0725-LY1054)

Name: Rabbit Anti-MADCAM1 Recombinant Antibody (12B5)
SKU: V2LY-0725-LY1054
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-MADCAM1 Recombinant Antibody (12B5) (V2LY-0725-LY1054) in FC

Overlay Peak curve showing PC3 cells surface stained with V2LY-0725-LY1054 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

12B5

Application

ELISA, FC

Immunogen

A synthesized peptide derived from human MADCAM1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Mucosal Vascular Addressin Cell Adhesion Molecule 1

Entrez Gene ID

8174

UniProt ID

Q13477

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Biological Process

Cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Cell-matrix adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of leukocyte migrationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of lymphocyte migrationIEA:InterPro
Receptor clusteringManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Membrane

Topology

Extracellular: 19-317
Helical: 318-338
Cytoplasmic: 339-382

PTM

The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2023). MAdCAM‐1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals. International Journal of Cancer.

Chelvanambi, M., & Wargo, J. A. (2023). MAdCAM-1: a newly identified microbial'gut check'for T cells. Trends in Immunology.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2022). Targeting MAdCAM-1 can prevent colitic cancer progression by suppressing immune cell infiltration and inflammatory signals. medRxiv, 2022-12.

Denton, A. E., Dooley, J., Cinti, I., Silva-Cayetano, A., Fra-Bido, S., Innocentin, S., ... & Linterman, M. A. (2022). Targeting TLR4 during vaccination boosts MAdCAM-1+ lymphoid stromal cell activation and promotes the aged germinal center response. Science immunology, 7(71), eabk0018.

Reinisch, W., Sandborn, W. J., Danese, S., Hébuterne, X., Kłopocka, M., Tarabar, D., ... & Vermeire, S. (2021). Long-term safety and efficacy of the Anti-MAdCAM-1 monoclonal antibody ontamalimab [SHP647] for the treatment of ulcerative colitis: the open-label study TURANDOT II. Journal of Crohn's and Colitis, 15(6), 938-949.

Schippers, A., Hübel, J., Heymann, F., Clahsen, T., Eswaran, S., Schlepütz, S., ... & Wagner, N. (2021). MAdCAM-1/α4β7 integrin-mediated lymphocyte/endothelium interactions exacerbate acute immune-mediated hepatitis in mice. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1227-1250.

Truffi, M., Sevieri, M., Morelli, L., Monieri, M., Mazzucchelli, S., Sorrentino, L., ... & Corsi, F. (2020). Anti-MAdCAM-1-conjugated nanocarriers delivering quantum dots enable specific imaging of inflammatory bowel disease. International Journal of Nanomedicine, 8537-8552.

Natoni, A., Farrell, M. L., Harris, S., Falank, C., Kirkham-McCarthy, L., Macauley, M. S., ... & O’Dwyer, M. (2020). Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model. Haematologica, 105(2), 457.

Tian, Y., Guo, Y., Zhu, P., Zhang, D., Liu, S., Tang, M., ... & Zhu, X. (2019). TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1. Aging (Albany NY), 11(19), 8623.

Kuhbandner, K., Hammer, A., Haase, S., Terbrack, E., Hoffmann, A., Schippers, A., ... & Stüve, O. (2019). MAdCAM-1-mediated intestinal lymphocyte homing is critical for the development of active experimental autoimmune encephalomyelitis. Frontiers in immunology, 10, 903.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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