Mouse Recombinant MADCAM1 protein, ECD, His Tag (V2LY-0526-LY8637)

Name: Mouse Recombinant MADCAM1 protein, ECD, His Tag
SKU: V2LY-0526-LY8637
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant MADCAM1 protein, ECD, His Tag consist of Amino Acid: 1-364 and predicts a molecular mass of 38.25 kDa.

Molecule Mass

38.25 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-364

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Mucosal Vascular Addressin Cell Adhesion Molecule 1

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Biological Process

Cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Cell-matrix adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of leukocyte migrationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of lymphocyte migrationIEA:InterPro
Receptor clusteringManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Membrane

Topology

Extracellular: 19-317
Helical: 318-338
Cytoplasmic: 339-382

PTM

The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2023). MAdCAM‐1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals. International Journal of Cancer.

Chelvanambi, M., & Wargo, J. A. (2023). MAdCAM-1: a newly identified microbial'gut check'for T cells. Trends in Immunology.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2022). Targeting MAdCAM-1 can prevent colitic cancer progression by suppressing immune cell infiltration and inflammatory signals. medRxiv, 2022-12.

Denton, A. E., Dooley, J., Cinti, I., Silva-Cayetano, A., Fra-Bido, S., Innocentin, S., ... & Linterman, M. A. (2022). Targeting TLR4 during vaccination boosts MAdCAM-1+ lymphoid stromal cell activation and promotes the aged germinal center response. Science immunology, 7(71), eabk0018.

Reinisch, W., Sandborn, W. J., Danese, S., Hébuterne, X., Kłopocka, M., Tarabar, D., ... & Vermeire, S. (2021). Long-term safety and efficacy of the Anti-MAdCAM-1 monoclonal antibody ontamalimab [SHP647] for the treatment of ulcerative colitis: the open-label study TURANDOT II. Journal of Crohn's and Colitis, 15(6), 938-949.

Schippers, A., Hübel, J., Heymann, F., Clahsen, T., Eswaran, S., Schlepütz, S., ... & Wagner, N. (2021). MAdCAM-1/α4β7 integrin-mediated lymphocyte/endothelium interactions exacerbate acute immune-mediated hepatitis in mice. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1227-1250.

Truffi, M., Sevieri, M., Morelli, L., Monieri, M., Mazzucchelli, S., Sorrentino, L., ... & Corsi, F. (2020). Anti-MAdCAM-1-conjugated nanocarriers delivering quantum dots enable specific imaging of inflammatory bowel disease. International Journal of Nanomedicine, 8537-8552.

Natoni, A., Farrell, M. L., Harris, S., Falank, C., Kirkham-McCarthy, L., Macauley, M. S., ... & O’Dwyer, M. (2020). Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model. Haematologica, 105(2), 457.

Tian, Y., Guo, Y., Zhu, P., Zhang, D., Liu, S., Tang, M., ... & Zhu, X. (2019). TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1. Aging (Albany NY), 11(19), 8623.

Kuhbandner, K., Hammer, A., Haase, S., Terbrack, E., Hoffmann, A., Schippers, A., ... & Stüve, O. (2019). MAdCAM-1-mediated intestinal lymphocyte homing is critical for the development of active experimental autoimmune encephalomyelitis. Frontiers in immunology, 10, 903.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MADCAM1 protein, ECD, His Tag (CAT#: V2LY-0526-LY5414)

Human Recombinant MADCAM1 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY5415)

Human MADCAM1 ELISA Kit (CAT#: V2LY-0626-LY681)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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