Sign in or Register   Sign in or Register
  |  

Rat Anti-MADCAM1 Monoclonal Antibody (MECA-367) (CBMAB-1382-YC)

Provided herein is a rat monoclonal antibody against Mouse Madcam-1. The antibody, clone MECA-367, can be used for immunoassay techniques, such as Block, FC, IHC-Fr, IP and WB.
See all MADCAM1 antibodies
Published Data
Fig.1 MAdCAM-1 in tumors from Treg depleted mice.
...View More
Fig. 1 Flow cytometry
...View More
Fig. 2 Immunofluorescent microscopy
...View More

Summary

Host Animal
Rat
Specificity
Mouse
Clone
MECA-367
Antibody Isotype
IgG2a
Application
Blocking, FC, IHC-Fr, IP, WB

Basic Information

Immunogen
Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Mucosal Vascular Addressin Cell Adhesion Molecule 1
Introduction
MADCAM1 is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. MADCAM1 is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1.
Entrez Gene ID
17123
UniProt ID
Q61826
Alternative Names
AV211525; MAdCAM-1
Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Biological Process
Cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Cell-matrix adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of leukocyte migrationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of lymphocyte migrationIEA:InterPro
Receptor clusteringManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Membrane
Topology
Extracellular: 19-317
Helical: 318-338
Cytoplasmic: 339-382
PTM
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2023). MAdCAM‐1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals. International Journal of Cancer.

Chelvanambi, M., & Wargo, J. A. (2023). MAdCAM-1: a newly identified microbial'gut check'for T cells. Trends in Immunology.

Ozawa, N., Yokobori, T., Osone, K., Bilguun, E. O., Okami, H., Shimoda, Y., ... & Saeki, H. (2022). Targeting MAdCAM-1 can prevent colitic cancer progression by suppressing immune cell infiltration and inflammatory signals. medRxiv, 2022-12.

Denton, A. E., Dooley, J., Cinti, I., Silva-Cayetano, A., Fra-Bido, S., Innocentin, S., ... & Linterman, M. A. (2022). Targeting TLR4 during vaccination boosts MAdCAM-1+ lymphoid stromal cell activation and promotes the aged germinal center response. Science immunology, 7(71), eabk0018.

Reinisch, W., Sandborn, W. J., Danese, S., Hébuterne, X., Kłopocka, M., Tarabar, D., ... & Vermeire, S. (2021). Long-term safety and efficacy of the Anti-MAdCAM-1 monoclonal antibody ontamalimab [SHP647] for the treatment of ulcerative colitis: the open-label study TURANDOT II. Journal of Crohn's and Colitis, 15(6), 938-949.

Schippers, A., Hübel, J., Heymann, F., Clahsen, T., Eswaran, S., Schlepütz, S., ... & Wagner, N. (2021). MAdCAM-1/α4β7 integrin-mediated lymphocyte/endothelium interactions exacerbate acute immune-mediated hepatitis in mice. Cellular and Molecular Gastroenterology and Hepatology, 11(4), 1227-1250.

Truffi, M., Sevieri, M., Morelli, L., Monieri, M., Mazzucchelli, S., Sorrentino, L., ... & Corsi, F. (2020). Anti-MAdCAM-1-conjugated nanocarriers delivering quantum dots enable specific imaging of inflammatory bowel disease. International Journal of Nanomedicine, 8537-8552.

Natoni, A., Farrell, M. L., Harris, S., Falank, C., Kirkham-McCarthy, L., Macauley, M. S., ... & O’Dwyer, M. (2020). Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model. Haematologica, 105(2), 457.

Tian, Y., Guo, Y., Zhu, P., Zhang, D., Liu, S., Tang, M., ... & Zhu, X. (2019). TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1. Aging (Albany NY), 11(19), 8623.

Kuhbandner, K., Hammer, A., Haase, S., Terbrack, E., Hoffmann, A., Schippers, A., ... & Stüve, O. (2019). MAdCAM-1-mediated intestinal lymphocyte homing is critical for the development of active experimental autoimmune encephalomyelitis. Frontiers in immunology, 10, 903.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rat Anti-MADCAM1 Monoclonal Antibody (MECA-367)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare