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Mouse Anti-MANF (AA 116-185) Recombinant Antibody (CBFYM-1368) (CBMAB-M1527-FY)

This product is mouse antibody that recognizes MANF. The antibody CBFYM-1368 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all MANF antibodies
Published Data
Fig.2 Western blot shows the expression of unpurified and purified MANF after elution with 40 mM of imidazole.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1368
Antibody Isotype
IgG1, k
Application
ELISA, IF, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: DSQICELKYD KQIDLSTVDL KKLRVKELKK ILDDWGETCK GCAEKSDYIR KINELMPKYA PKAASARTDL
Specificity
Human
Antibody Isotype
IgG1, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 116-185

Target

Full Name
MESENCEPHALIC ASTROCYTE DERIVED NEUROTROPHIC FACTOR
Introduction
The protein encoded by this gene is localized in the endoplasmic reticulum and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.
Entrez Gene ID
7873
UniProt ID
P55145
Alternative Names
Mesencephalic Astrocyte Derived Neurotrophic Factor; Mesencephalic Astrocyte-Derived Neurotrophic Factor; Arginine-Rich, Mutated In Early Stage Tumors; ARMET; ARP; Arginine-Rich Protein; Protein ARMET
Function
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311).
Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity).
Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity).
Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057).
Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475).
Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475).
Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057).
Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057).
Biological Process
Dopaminergic neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Neuron projection developmentManual Assertion Based On ExperimentIBA:GO_Central
Regulation of response to endoplasmic reticulum stressManual Assertion Based On ExperimentIMP:UniProtKB
Response to unfolded proteinIEA:UniProtKB-KW
Cellular Location
Secreted
Endoplasmic reticulum lumen
Sarcoplasmic reticulum lumen
Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475).
Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475, PubMed:29497057).
PTM
May contain sialic acid residues.

Wen, W., Wang, Y., Li, H., Hu, D., Zhang, Z., Lin, H., & Luo, J. (2023). Upregulation of mesencephalic astrocyte‐derived neurotrophic factor (MANF) expression offers protection against alcohol neurotoxicity. Journal of neurochemistry, 166(6), 943-959.

Zhang, J. X., Zhou, K. G., Yin, Y. X., Jin, L. J., Tong, W. F., Guo, J., ... & Jiang, M. (2023). Mesencephalic astrocyte-derived neurotrophic factor (MANF) prevents the neuroinflammation induced dopaminergic neurodegeneration. Experimental Gerontology, 171, 112037.

Sivakumar, B., & Krishnan, A. (2023). Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF): An Emerging Therapeutic Target for Neurodegenerative Disorders. Cells, 12(7), 1032.

Liu, Y. Y., Huo, D., Zeng, L. T., Fan, G. Q., Shen, T., Zhang, T. M., ... & Cui, J. (2022). Mesencephalic astrocyte-derived neurotrophic factor (MANF): Structure, functions and therapeutic potential. Ageing Research Reviews, 101763.

Yang, F., Qu, Y., Yan, Z., Wang, D., Li, W., & Yao, L. (2022). Increased serum concentrations of Mesencephalic astrocyte-derived neurotrophic factor in patients and rats with ischemic stroke. Journal of Stroke and Cerebrovascular Diseases, 31(11), 106752.

Chhetri, G., Liang, Y., Shao, J., Han, D., Yang, Y., Hou, C., ... & Shen, Y. (2020). Role of mesencephalic astrocyte-derived neurotrophic factor in alcohol-induced liver injury. Oxidative medicine and cellular longevity, 2020.

Wen, W., Wang, Y., Li, H., Xu, H., Xu, M., Frank, J. A., ... & Luo, J. (2020). Mesencephalic astrocyte-derived neurotrophic factor (MANF) regulates neurite outgrowth through the activation of Akt/mTOR and Erk/mTOR signaling pathways. Frontiers in molecular neuroscience, 13, 560020.

Galli, E., Planken, A., Kadastik-Eerme, L., Saarma, M., Taba, P., & Lindholm, P. (2019). Increased serum levels of mesencephalic astrocyte-derived neurotrophic factor in subjects with Parkinson’s disease. Frontiers in Neuroscience, 13, 929.

Xu, S., Di, Z., He, Y., Wang, R., Ma, Y., Sun, R., ... & Shen, Y. (2019). Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stress. Journal of Neuroinflammation, 16, 1-14.

Danilova, T., Galli, E., Pakarinen, E., Palm, E., Lindholm, P., Saarma, M., & Lindahl, M. (2019). Mesencephalic astrocyte-derived neurotrophic factor (MANF) is highly expressed in mouse tissues with metabolic function. Frontiers in endocrinology, 10, 765.

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For research use only. Not intended for any clinical use.

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