Human Recombinant MANF protein, hFc Tag (V2LY-0526-LY5423)

Name: Human Recombinant MANF protein, hFc Tag
SKU: V2LY-0526-LY5423
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant MANF protein, hFc Tag consist of Amino Acid: 1-182 and predicts a molecular mass of 44.9 kDa.

Molecule Mass

44.9 kDa

Sequence

Amino Acid: 1-182

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MESENCEPHALIC ASTROCYTE DERIVED NEUROTROPHIC FACTOR

Function

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311).
Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity).
Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity).
Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057).
Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475).
Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475).
Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057).
Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057).

Biological Process

Dopaminergic neuron differentiationManual Assertion Based On ExperimentIBA:GO_Central
Neuron projection developmentManual Assertion Based On ExperimentIBA:GO_Central
Regulation of response to endoplasmic reticulum stressManual Assertion Based On ExperimentIMP:UniProtKB
Response to unfolded proteinIEA:UniProtKB-KW

Cellular Location

Secreted
Endoplasmic reticulum lumen
Sarcoplasmic reticulum lumen
Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475).
Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475, PubMed:29497057).

PTM

May contain sialic acid residues.

Wen, W., Wang, Y., Li, H., Hu, D., Zhang, Z., Lin, H., & Luo, J. (2023). Upregulation of mesencephalic astrocyte‐derived neurotrophic factor (MANF) expression offers protection against alcohol neurotoxicity. Journal of neurochemistry, 166(6), 943-959.

Zhang, J. X., Zhou, K. G., Yin, Y. X., Jin, L. J., Tong, W. F., Guo, J., ... & Jiang, M. (2023). Mesencephalic astrocyte-derived neurotrophic factor (MANF) prevents the neuroinflammation induced dopaminergic neurodegeneration. Experimental Gerontology, 171, 112037.

Sivakumar, B., & Krishnan, A. (2023). Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF): An Emerging Therapeutic Target for Neurodegenerative Disorders. Cells, 12(7), 1032.

Liu, Y. Y., Huo, D., Zeng, L. T., Fan, G. Q., Shen, T., Zhang, T. M., ... & Cui, J. (2022). Mesencephalic astrocyte-derived neurotrophic factor (MANF): Structure, functions and therapeutic potential. Ageing Research Reviews, 101763.

Yang, F., Qu, Y., Yan, Z., Wang, D., Li, W., & Yao, L. (2022). Increased serum concentrations of Mesencephalic astrocyte-derived neurotrophic factor in patients and rats with ischemic stroke. Journal of Stroke and Cerebrovascular Diseases, 31(11), 106752.

Chhetri, G., Liang, Y., Shao, J., Han, D., Yang, Y., Hou, C., ... & Shen, Y. (2020). Role of mesencephalic astrocyte-derived neurotrophic factor in alcohol-induced liver injury. Oxidative medicine and cellular longevity, 2020.

Wen, W., Wang, Y., Li, H., Xu, H., Xu, M., Frank, J. A., ... & Luo, J. (2020). Mesencephalic astrocyte-derived neurotrophic factor (MANF) regulates neurite outgrowth through the activation of Akt/mTOR and Erk/mTOR signaling pathways. Frontiers in molecular neuroscience, 13, 560020.

Galli, E., Planken, A., Kadastik-Eerme, L., Saarma, M., Taba, P., & Lindholm, P. (2019). Increased serum levels of mesencephalic astrocyte-derived neurotrophic factor in subjects with Parkinson’s disease. Frontiers in Neuroscience, 13, 929.

Xu, S., Di, Z., He, Y., Wang, R., Ma, Y., Sun, R., ... & Shen, Y. (2019). Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stress. Journal of Neuroinflammation, 16, 1-14.

Danilova, T., Galli, E., Pakarinen, E., Palm, E., Lindholm, P., Saarma, M., & Lindahl, M. (2019). Mesencephalic astrocyte-derived neurotrophic factor (MANF) is highly expressed in mouse tissues with metabolic function. Frontiers in endocrinology, 10, 765.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MANF protein, His Tag (CAT#: V2LY-0526-LY5424)

Mouse Recombinant MANF protein, His Tag (CAT#: V2LY-0526-LY8640)

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Mouse Recombinant MANF protein, His Tag (CAT#: V2LY-0526-LY8640)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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