Mouse Anti-MAP2K2 Recombinant Antibody (CBT880) (V2LY-0625-LY3204)

Name: Mouse Anti-MAP2K2 Recombinant Antibody (CBT880)
SKU: V2LY-0625-LY3204
Rating: 5 (1 reviews)

See all MAP2K2 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-MAP2K2 Recombinant Antibody (CBT880) (V2LY-0625-LY3204) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT880

Application

Immunogen

Synthesized peptide derived from human MEK-2 around the non-phosphorylation site of T394.

Host Species

Mouse

Specificity

Human, Rat, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase Kinase 2

Entrez Gene ID

5605

UniProt ID

P36507

Alternative Names

Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2;

Function

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).

Biological Process

ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Peptidyl-serine autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of production of miRNAs involved in gene silencing by miRNAManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of early endosome to late endosome transportManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of Golgi inheritanceManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of stress-activated MAPK cascadeManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Cytoplasm
Membrane
Membrane localization is probably regulated by its interaction with KSR1.

Involvement in disease

Cardiofaciocutaneous syndrome 4 (CFC4):
A form of cardiofaciocutaneous syndrome, a multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices.

PTM

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).
(Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Sarkar, A., Chamucero-Millares, J. A., & Rojas, M. (2022). Romulus and Remus of Inflammation: The Conflicting Roles of MAP2K1 and MAP2K2 in Acute Respiratory Distress Syndrome. American Journal of Respiratory Cell and Molecular Biology, 66(5), 479-480.

Hua, Z., Wei, R., Guo, M., Lin, Z., Yu, X., Li, X., ... & Yang, Y. (2022). YTHDF2 promotes multiple myeloma cell proliferation via STAT5A/MAP2K2/p-ERK axis. Oncogene, 41(10), 1482-1491.

Gong, K. Q., Mikacenic, C., Long, M. E., Frevert, C. W., Birkland, T. P., Charron, J., ... & Manicone, A. M. (2022). MAP2K2 delays recovery in murine models of acute lung injury and associates with acute respiratory distress syndrome outcome. American Journal of Respiratory Cell and Molecular Biology, 66(5), 555-563.

Ren, L. X., Qi, J. C., Zhao, A. N., Shi, B., Zhang, H., Wang, D. D., & Yang, Z. (2021). Myc-associated zinc-finger protein promotes clear cell renal cell carcinoma progression through transcriptional activation of the MAP2K2-dependent ERK pathway. Cancer cell international, 21(1), 1-13.

Xu, J., Xiong, H., Zhao, Z., Luo, M., Ju, Y., Yang, G., & Mei, Z. (2021). Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway. Food & Function, 12(10), 4556-4569.

Jacinto, J. G., Häfliger, I. M., Gentile, A., & Drögemüller, C. (2021). A heterozygous missense variant in MAP2K2 in a stillborn romagnola calf with skeletal-cardio-enteric dysplasia. Animals, 11(7), 1931.

Richmond, C. S., Vallatharasu, Y., Deviley, J. A., Vos, C. R., Parsons, B. M., & Kenny, P. A. (2019). Sequential treatment failures in response to BRAF/MEK and immune checkpoint inhibitors mediated by MAP2K2 and B2M mutations in melanoma. Experimental and Molecular Pathology, 110, 104260.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-MAP2K2 Recombinant Antibody (CBT880)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MAP2K2 Matched Antibody Pair (712) (CAT#: APMAB-712LY)

Related Products

Rabbit Anti-MAP2K2 (C-terminus) Recombinant Antibody (CBFYM-1452) (CAT#: CBMAB-M1612-FY)

Mouse Anti-MAP2K2 Recombinant Antibody (CBFYM-1451) (CAT#: CBMAB-M1611-FY)

Human Recombinant MEK2 protein, GST Tag-1 (CAT#: V2LY-0526-LY5524)

Mouse Anti-MAP2K2 Recombinant Antibody (CBFYM-0162) (CAT#: CBMAB-M0184-FY)

Human Recombinant MEK2, Active protein, GST Tag (CAT#: V2LY-0526-LY5522)

Rabbit Anti-MAP2K2 Recombinant Antibody (CBFYM-1455) (CAT#: CBMAB-M1615-FY)

Rabbit Anti-MAP2K2 Recombinant Antibody (A994) (CAT#: CBMAB-AP11660LY)

MAP2K2 Matched Antibody Pair (712) (CAT#: APMAB-712LY)

Mouse Anti-MAP2K2 Recombinant Antibody (CB307) (CAT#: CBMAB-CS307LY)

Rabbit Anti-MAP2K2 Recombinant Antibody (CBFYM-0451) (CAT#: CBMAB-M0569-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry