Rabbit Anti-MAP4K4 (C-terminus) Monoclonal Antibody (CBFYH-3293) (CBMAB-H3679-FY)

Name: Rabbit Anti-MAP4K4 (C-terminus) Monoclonal Antibody (CBFYH-3293)
SKU: CBMAB-H3679-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYH-3293

Application

IHC, IHC-P

Immunogen

Synthetic peptide derived from the C-terminus of human MAP4K4 protein

Specificity

Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

C-terminus

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4

Introduction

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Entrez Gene ID

9448

UniProt ID

O95819

Alternative Names

Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4; Hepatocyte Progenitor Kinase-Like/Germinal Center Kinase-Like Kinase; MAPK/ERK Kinase Kinase Kinase 4; HPK/GCK-Like Kinase HGK; Nck-Interacting Kinase; MEK Kinase Kinase 4; EC 2.7.11.1; NIK; HGK; Epididymis Secretory Protein Li 31

Function

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.

Biological Process

Intracellular signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:CACAO
Negative regulation of cell-matrix adhesion1 PublicationNAS:ARUK-UCL
Neuron projection morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of ARF protein signal transductionBy SimilarityISS:ARUK-UCL
Positive regulation of cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of focal adhesion assemblyBy SimilarityISS:ARUK-UCL
Positive regulation of focal adhesion disassemblyBy SimilarityISS:ARUK-UCL
Positive regulation of GTPase activityBy SimilarityISS:ARUK-UCL
Positive regulation of keratinocyte migrationBy SimilarityISS:ARUK-UCL
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of JNK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm

Patterson, V., Ullah, F., Bryant, L., Li, D., Griffin, J. N., Sidhu, A., ... & Bhoj, E. (2023). Abrogation of MAP4K4 protein function causes congenital anomalies in humans and zebrafish. Science advances, 9(17), eade0631.

González-Montero, J., Rojas, C. I., & Burotto, M. (2023). MAP4K4 and cancer: ready for the main stage?. Frontiers in Oncology, 13, 1162835.

Singh, S. K., Roy, R., Kumar, S., Srivastava, P., Jha, S., Rana, B., & Rana, A. (2023). Molecular Insights of MAP4K4 Signaling in Inflammatory and Malignant Diseases. Cancers, 15(8), 2272.

Jovanovic, D., Yan, S., & Baumgartner, M. (2022). The molecular basis of the dichotomous functionality of MAP4K4 in proliferation and cell motility control in cancer. Frontiers in oncology, 12, 1059513.

Zhang, J., Cai, X., Cui, W., & Wei, Z. (2022). Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer. Genes, 13(10), 1786.

Singh, S. K., Kumar, S., Viswakarma, N., Principe, D. R., Das, S., Sondarva, G., ... & Rana, A. (2021). MAP4K4 promotes pancreatic tumorigenesis via phosphorylation and activation of mixed lineage kinase 3. Oncogene, 40(43), 6153-6165.

Vu, L. D., Xu, X., Zhu, T., Pan, L., van Zanten, M., de Jong, D., ... & De Smet, I. (2021). The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis. Nature Communications, 12(1), 2842.

Fuller, S. J., Edmunds, N. S., McGuffin, L. J., Hardyman, M. A., Cull, J. J., Alharbi, H. O., ... & Clerk, A. (2021). MAP4K4 expression in cardiomyocytes: multiple isoforms, multiple phosphorylations and interactions with striatins. Biochemical Journal, 478(11), 2121-2143.

Fiedler, L. R., Chapman, K., Xie, M., Maifoshie, E., Jenkins, M., Golforoush, P. A., ... & Schneider, M. D. (2019). MAP4K4 inhibition promotes survival of human stem cell-derived cardiomyocytes and reduces infarct size in vivo. Cell Stem Cell, 24(4), 579-591.

Wu, C., Watts, M. E., & Rubin, L. L. (2019). MAP4K4 activation mediates motor neuron degeneration in amyotrophic lateral sclerosis. Cell reports, 26(5), 1143-1156.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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