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Mouse Anti-MAP4K4 (AA 611-710) Recombinant Antibody (CBFYM-1552) (CBMAB-M1718-FY)

This product is mouse antibody that recognizes MAP4K4. The antibody CBFYM-1552 can be used for immunoassay techniques such as: ELISA, WB.
See all MAP4K4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1552
Antibody Isotype
IgG2a, k
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: VHPALQRPAE PQVQWSHLAS LKNNVSPVSR SHSFSDPSPK FAHHHLRSQD PCPPSRSEVL SQSSDSKSEA PDPTQKAWSR SDSDEVPPRV PVRTTSRSPV
Specificity
Human
Antibody Isotype
IgG2a, k
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 611-710

Target

Full Name
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Introduction
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Entrez Gene ID
9448
UniProt ID
O95819
Alternative Names
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4; Hepatocyte Progenitor Kinase-Like/Germinal Center Kinase-Like Kinase; MAPK/ERK Kinase Kinase Kinase 4; HPK/GCK-Like Kinase HGK; Nck-Interacting Kinase; MEK Kinase Kinase 4; EC 2.7.11.1; NIK; HGK; Epididymis Secretory Protein Li 31
Function
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Biological Process
Intracellular signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:CACAO
Negative regulation of cell-matrix adhesion1 PublicationNAS:ARUK-UCL
Neuron projection morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of ARF protein signal transductionBy SimilarityISS:ARUK-UCL
Positive regulation of cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of focal adhesion assemblyBy SimilarityISS:ARUK-UCL
Positive regulation of focal adhesion disassemblyBy SimilarityISS:ARUK-UCL
Positive regulation of GTPase activityBy SimilarityISS:ARUK-UCL
Positive regulation of keratinocyte migrationBy SimilarityISS:ARUK-UCL
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of JNK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm

Patterson, V., Ullah, F., Bryant, L., Li, D., Griffin, J. N., Sidhu, A., ... & Bhoj, E. (2023). Abrogation of MAP4K4 protein function causes congenital anomalies in humans and zebrafish. Science advances, 9(17), eade0631.

González-Montero, J., Rojas, C. I., & Burotto, M. (2023). MAP4K4 and cancer: ready for the main stage?. Frontiers in Oncology, 13, 1162835.

Singh, S. K., Roy, R., Kumar, S., Srivastava, P., Jha, S., Rana, B., & Rana, A. (2023). Molecular Insights of MAP4K4 Signaling in Inflammatory and Malignant Diseases. Cancers, 15(8), 2272.

Jovanovic, D., Yan, S., & Baumgartner, M. (2022). The molecular basis of the dichotomous functionality of MAP4K4 in proliferation and cell motility control in cancer. Frontiers in oncology, 12, 1059513.

Zhang, J., Cai, X., Cui, W., & Wei, Z. (2022). Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer. Genes, 13(10), 1786.

Singh, S. K., Kumar, S., Viswakarma, N., Principe, D. R., Das, S., Sondarva, G., ... & Rana, A. (2021). MAP4K4 promotes pancreatic tumorigenesis via phosphorylation and activation of mixed lineage kinase 3. Oncogene, 40(43), 6153-6165.

Vu, L. D., Xu, X., Zhu, T., Pan, L., van Zanten, M., de Jong, D., ... & De Smet, I. (2021). The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis. Nature Communications, 12(1), 2842.

Fuller, S. J., Edmunds, N. S., McGuffin, L. J., Hardyman, M. A., Cull, J. J., Alharbi, H. O., ... & Clerk, A. (2021). MAP4K4 expression in cardiomyocytes: multiple isoforms, multiple phosphorylations and interactions with striatins. Biochemical Journal, 478(11), 2121-2143.

Fiedler, L. R., Chapman, K., Xie, M., Maifoshie, E., Jenkins, M., Golforoush, P. A., ... & Schneider, M. D. (2019). MAP4K4 inhibition promotes survival of human stem cell-derived cardiomyocytes and reduces infarct size in vivo. Cell Stem Cell, 24(4), 579-591.

Wu, C., Watts, M. E., & Rubin, L. L. (2019). MAP4K4 activation mediates motor neuron degeneration in amyotrophic lateral sclerosis. Cell reports, 26(5), 1143-1156.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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